Sequencing and bacterial expression of a novel murine alpha interferon gene

A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed in Escherichia coli under the control of the PL promoter which resulted in antiviral activity on mouse L-cel...

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Published inScience China. Life sciences Vol. 40; no. 3; pp. 277 - 283
Main Author 王焱 王征宇 周鸣南 蔡菊娥 孙兰英 刘新垣 B.L.Daugherty S.Pestka
Format Journal Article
LanguageEnglish
Published China Springer Nature B.V 01.06.1997
Subjects
alpha
Antiviral activity
expression
Genes
Interferon
method
murine
Nucleotide sequence
primer-based
prokaryotic
sequencing
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ISSN1674-7305
1006-9305
1869-1889
1862-2798
DOI10.1007/BF02879088

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Summary:A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed in Escherichia coli under the control of the PL promoter which resulted in antiviral activity on mouse L-cells. The sequence of mlFN-αB has been accepted by GENEBANK.
Bibliography:WANG Yan I WANG Zhengyu , ZHOU MingnanCAIJu’e SUN Lanying LIU Xinyuan(Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai 200031, China)B.L. Daugherty and S. Pestka(Department of Molecular Genetics and Microbiology, UMDNJ-Robert Wood Johnson Medical School, New Jersey, USA)
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ISSN:1674-7305
1006-9305
1869-1889
1862-2798
DOI:10.1007/BF02879088