Effect of solution viscosity on retardation of cell sedimentation in DLP 3D printing of gelatin methacrylate/silk fibroin bioink

• Published in: Journal of industrial and engineering chemistry (Seoul, Korea) Vol. 61; pp. 340 - 347
• Main Authors: Na, Kyunga, Shin, Sungchul, Lee, Hyunji, Shin, Donghyeok, Baek, Jihye, Kwak, Hojung, Park, Minsung, Shin, Jonghyun, Hyun, Jinho
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 25.05.2018; 한국공업화학회
• Subjects: Cell sedimentation; Digital light processing (DLP) 3D printing; Gelatin-methacrylate (GelMA); Silk fibroin particle; Solution viscosity; 화학공학; Solution viscosity; Silk fibroin particle; Cell sedimentation; Digital light processing (DLP) 3D printing; Gelatin-methacrylate (GelMA)
• ISSN: 1226-086X; 1876-794X
• DOI: 10.1016/j.jiec.2017.12.032

[Display omitted] To improve the cell suspension of the gelatin-methacrylate(GelMA) bioink, the viscosity of the ink solution was controlled by biocompatible silk fibroin(SF) particles. The viscosity of the GelMA solution increased about 1.4 × 102 folds by the addition of 1 w/v% SF particles. The increase of solution viscosity reduced the displacement of cells and retarded the cell sedimentation significantly. SF-GelMA bioink containing 1 w/v% SF was non-cytotoxic and used for the 3D bioprinting by directly projecting a feature onto the bioink. The cells encapsulated in the structures showed a high level of metabolic activity and demonstrated that SF-GelMA was a biocompatible material.