Alteration in endometrial helper T‐cell subgroups in chronic endometritis

• Published in: American journal of reproductive immunology (1989) Vol. 85; no. 3; pp. e13372 - n/a
• Main Authors: Kitazawa, Jun, Kimura, Fuminori, Nakamura, Akiko, Morimune, Aina, Hanada, Tetsuro, Amano, Tsukuru, Tsuji, Shunichiro, Kasahara, Kyoko, Satooka, Hiroki, Hirata, Takako, Kushima, Ryoji, Murakami, Takashi
• Format: Journal Article
• Language: English
• Published: Denmark Wiley Subscription Services, Inc 01.03.2021
• Subjects: CD4 antigen; chronic endometritis; Endometritis; Endometrium; Flow cytometry; Foxp3 protein; Helper cells; helper T cell; Immunofluorescence; Immunoregulation; implantation; infertility; Localization; Lymphocytes T; Th1/Th2/Th17/Treg; implantation; helper T cell; infertility; Th1/Th2/Th17/Treg; chronic endometritis
• ISSN: 1046-7408; 1600-0897; 1600-0897
• DOI: 10.1111/aji.13372

Problem The effect of chronic endometritis (CE) on the subpopulation of CD4+ T cells, Th1, Th2, Th17, and regulatory T cells in the endometrium is unknown. Method of study Lymphocytes were isolated from the endometrium of CE patients (n = 12) and non‐CE patients (n = 7). The CD4+ T‐cell profile was analyzed by flow cytometry and immunofluorescence. Results In the endometrium of CE patients, there were significantly more Th1 cells among CD4+ cells and fewer Th2 cells in comparison to non‐CE patients. No marked difference was observed in Th17 cells or Foxp3+ Treg cells. Moreover, the proportion of Th1 cells increased and the proportion of Th2 cells decreased as the number of CD138+ cells increased. Furthermore, when the localization of CD138+ cells and CD4+ cells was examined, CD4+ cells were found to be clustered around CD138+ cells in CE patients. Conclusion The CD4+ T‐cell profile in the endometrium is altered in women with CE. This finding may help to clarify the pathophysiology and development of treatment methods for CE.