Effects of hyaluronic acid on Ca2+ influx, lactate dehydrogenase activity, and cyclic AMP synthesis in canine ejaculated sperm during in vitro capacitation

• Published in: Journal of Veterinary Medical Science Vol. 68; no. 2; pp. 119 - 123
• Main Authors: Kawakami, E.(Nippon Veterinary and Animal Science Univ, Musashino, Tokyo (Japan)), Nishizono, Y, Miyata, C, Hirano, T, Hori, T, Tsutsui, T
• Format: Journal Article
• Language: English
• Published: Tokyo JAPANESE SOCIETY OF VETERINARY SCIENCE 2006; Japan Science and Technology Agency
• Subjects: ACIDE HYALURONIQUE; ACIDO HIALURONICO; Ca2+ influx; CALCIO; CALCIUM; canine; CHIEN; DOGS; ESPERMATOZOO; HYALURONIC ACID; LACTATE DEHYDROGENASE; LACTATE DESHYDROGENASE; LACTATO DESHIDROGENASA; METABOLISM; METABOLISME; METABOLISMO; PERRO; sperm capacitation; SPERMATOZOA; SPERMATOZOIDE
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.68.119

The effects of hyaluronic acid, which comprises the cumulus intercellular matrix, on CaE2+ influx, lactate dehydrogenase (LDH) activity, and cyclic AMP synthesis in canine sperm during capacitation was investigated. Ejaculated sperm were collected from 10 Beagle dogs and the sperm were incubated for 4 hr in Eagle's MEM containing 10 microg/ml of hyaluronic acid. The percentages of actively motile sperm, hyperactivated sperm (HA-sperm), acrosome-reacted sperm (AR-sperm), and sperm labeled with fluoresceinated CaE2+ indicator (CaE2+-labeled sperm) were evaluated to assess CaE2+ influx into the sperm. LDH activity and cAMP concentration were measured in homogenized sperm. The mean percentages of motile sperm, HA-sperm, and CaE2+-labeled sperm in the MEM containing hyaluronic acid were higher than in the control medium (P0.05, 0.05, and 0.01, respectively), but there was no difference between the percentages of AR-sperm. Mean LDH activity and mean cAMP concentration were also significantly higher than the control values (P0.05). The percentages of HA-sperm correlated with those of CaE2+-labeled sperm (rE2