Effects of 5-Aza-CdR on Cell Proliferation of Breast Cancer Cell Line MDA-MB-435S and Expression of maspin Gene

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 27; no. 5; pp. 543 - 546
• Main Author: 张波 黄韬 刘科 陈剑英 王国斌
• Format: Journal Article
• Language: English
• Published: China Department of General Surgery, Union Hospital, Tongfi Medical College, Huazhong University of Science and Technology, Wuhan 430030, China 01.10.2007
• Subjects: 5-Aza-CdR; Antimetabolites, Antineoplastic - pharmacology; Azacitidine - analogs & derivatives; Azacitidine - pharmacology; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cell Line, Tumor; Cell Proliferation - drug effects; DNA甲基化; Female; Humans; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Serpins - genetics; Serpins - metabolism; 乳腺癌; 基因表达; 细胞增殖; DNA methylation; breast cancer; 5-Aza-2'-deoxycytidine; maspin
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-007-0517-z

The effects of 5-Aza-2＇-deoxycytidine （5-Aza-CdR） on the proliferation of MDA-MB-435S cells and the expression of tumor suppressor gene maspin were investigated. Human breast cancer cell line MDA-MB-435S was treated with 5 μmol/L 5-Aza-CdR, a specific demethylating agent for 0 to 8 days. The growth of MDA-MB-435S cells was observed by MTT assay before and after 5-Aza-CdR treatment, respectively. The expression of maspin mRNA was detected by reverse transcfiption-polymerase chain reaction （RT-PCR）. The cell cycle of MDA-MB-435S cells was analyzed by flow cytometry. The results showed that the growth of MDA-MB-435S cells treated with 5-Aza-CdR for 8 days was significantly suppressed as compared with the control groups, and the inhibition rate increased sharply from 5 day to 8 day （35.42% to 71.29%）. Flow cytometry showed that 5 μmol/L 5-Aza-CdR could induce G2/M cell cycle arrest and decrease the percentage of mitosis cell number in this cell line. Maspin mRNA was expressed in MDA-MB-435S cells after 5-Aza-CdR treatment, but it was weakly detectable before the treatment. It was concluded that Maspin gene might be transcriptional silencing by hypermethylation and the re-expression of maspin gene by 5-Aza-CdR can inhibit the proliferation and induce the G2/M arrest of MDA-MB-435S breast cancer cells.