Isolation, Purification, and Structural Identification of an Antifungal Compound from a Trichoderma Strain

• Published in: Journal of microbiology and biotechnology Vol. 25; no. 8; pp. 1257 - 1264
• Main Authors: Li, Chong-Wei, Song, Rui-Qing, Yang, Li-Bin, Deng, Xun
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 01.08.2015
• Subjects: Antifungal Agents - chemistry; Antifungal Agents - isolation & purification; Chromatography, Liquid; Chromatography, Thin Layer; Gas Chromatography-Mass Spectrometry; Magnetic Resonance Spectroscopy; Microbial Sensitivity Tests; Molecular Structure; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet; Trichoderma - chemistry; 생물학; Trichoderma; antifungal; Cytospora chrysosperma; biocontrol; bioactive component; structural identification
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1410.10027

Trichoderma strain T-33 has been demonstrated to have inhibitory effect on the fungus species Cytospora chrysosperma. Here, an active antifungal compound was obtained from Trichoderma strain T-33 extract via combined separation technologies, including organic solvent extraction, liquid chromatography, and thin-layer chromatography. The purified compound was further characterized by advanced analytical technologies to elucidate its chemical structure. Results indicated that the active antifungal compound in Trichoderma strain T-33 extract is 2,5- cyclohexadiene-1,4-dione-2,6-bis (1,1-dimethylethyl).