The Effect of Nitrous Oxide and Isoflurane on the Total RNA Yield from the Cochlea of the Rats

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 27; no. 5; pp. 614 - 616
• Main Author: 李元涛 柯昌斌 杨京利 熊良志 姚尚龙
• Format: Journal Article
• Language: English
• Published: China Department of Anesthesiology, Affiliated Shiyan Taihe Hospital of Yunyang Medical College, Shiyan 442000, China%Department of Anesthesiology, Affiliated Shiyan Taihe Hospital of Yunyang Medical College, Shiyan 442000, China%Department of Anesthesiology, Union Hospital Tongji Medical College, Huazhong University of Science and Technology, Wnhan 430030, China 01.10.2007; Department of Anesthesiology, Union Hospital Tongji Medical College, Huazhong University of Science and Technology, Wnhan 430030, China
• Subjects: Anesthetics, Inhalation - adverse effects; Anesthetics, Inhalation - pharmacology; Animals; Cochlea - metabolism; Cochlea - pathology; Female; Isoflurane - adverse effects; Isoflurane - pharmacology; Male; Nitrous Oxide - adverse effects; Nitrous Oxide - pharmacology; Random Allocation; Rats; Rats, Wistar; RNA; RNA - analysis; 一氧化二氮; 动物模型; 麻醉剂; cochlea; isoflurane; nitrous oxide; total RNA; yield
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-007-0536-9

The possible mechanism of inhalation anesthetics on the internal auditory impairment of the rat was investigated by determining the effect of nitrous oxide （N2O） and isoflurane on the total RNA yield from the cochlea of the rats. Thirty healthy Wistar rats were randomly divided into 3 groups： group C （control group, n=10） with a 3-h unremitting inhalation of 50% 02, group N （experiment group, n= 10） with a continuous inhalation of 50% N20＋50% O2for 3 h, and group I （experiment group, n=10） with a 3-h sustained inhalation of 2.5% isoflurane. The TRIzol in combination with RNeasy was used to respectively extract the total RNA from cochlea of rats in the 3 groups. Spectrophotometry was used to detect total RNA yield and electrophoresis to detect the quality. The total RNA extracted from the cochlea of the rats in the groups C and N was 7.69 and 6.51 μg, respectively. There was a 15% decrease in the N group as compared with group C. The total RNA from the rats in the group I was 7.32μg, and there was hardly any change in the group as compared with the group C. The value of A260/A280 in groups C, N and I was 2.07, 2.04 and 2.04, respectively, showing a very high RNA purity. The result of gel electrophoresis suggested that there was no degradation in the total RNA. It was suggested that the interference of N20 on the cochlear RNA yield might be one of the reasons which cause an injury of the ear. The isoflurane shows no harm on the hearing.