Minimum spanning tree analysis of unimpaired individuals at risk of Alzheimer’s disease

• Published in: Brain communications Vol. 6; no. 5; p. fcae283
• Main Authors: García-Colomo, Alejandra, López-Sanz, David, Stam, Cornelis J, Hillebrand, Arjan, Carrasco-Gómez, Martín, Spuch, Carlos, Comis-Tuche, María, Maestú, Fernando
• Format: Journal Article
• Language: English
• Published: UK Oxford University Press 2024
• Subjects: Original; brain networks; magnetoencephalography; cognitively unimpaired; minimum spanning tree; p-tau231
• ISSN: 2632-1297; 2632-1297
• DOI: 10.1093/braincomms/fcae283

Abstract Identifying early and non-invasive biomarkers to detect individuals in the earliest stages of the Alzheimer’s disease continuum is crucial. As a result, electrophysiology and plasma biomarkers are emerging as great candidates in this pursuit due to their low invasiveness. This is the first magnetoencephalography study to assess the relationship between minimum spanning tree parameters, an alternative to overcome the comparability and thresholding problem issues characteristic of conventional brain network analyses, and plasma phosphorylated tau231 levels in unimpaired individuals, with different risk levels of Alzheimer’s disease. Seventy-six individuals with available magnetoencephalography recordings and phosphorylated tau231 plasma determination were included. The minimum spanning tree for the theta, alpha and beta bands for each subject was obtained, and the leaf fraction, tree hierarchy and diameter were calculated. To study the relationship between these topological parameters and phosphorylated tau231, we performed correlation analyses, for the whole sample and considering the two risk sub-groups separately. Increasing concentrations of phosphorylated tau231 were associated with greater leaf fraction and tree hierarchy values, along with lower diameter values, for the alpha and theta frequency bands. These results emerged for the whole sample and the higher risk group, but not for the lower risk group. Our results indicate that the network topology of cognitively unimpaired individuals with elevated plasma phosphorylated tau231 levels, a marker of Alzheimer’s disease pathology and amyloid-β accumulation, is already altered, shifting towards a more integrated network increasing its vulnerability and hub-dependency, mostly in the alpha band. This is indicated by increases in leaf fraction and tree hierarchy, along with reductions in diameter. These results match the initial trajectory proposed by theoretical models of disease progression and network disruption and suggest that changes in brain function and organization begin early on. García-Colomo et al. show an early topological network reorganization associated with the most incipient increases in the pathology marker p-tau231 in cognitively unimpaired individuals, especially relatives of Alzheimer’s disease. Elevated p-tau231 levels are associated with a star-like network topology. Graphical Abstract Graphical Abstract