Effects of porcine testis extract on wound healing in rat

• Published in: Animal cells and systems Vol. 16; no. 6; pp. 469 - 478
• Main Authors: Lee, D.M., Yeungnam University, Gyeongsan, Republic of Korea, Abdul Roouf Bhat, Yeungnam University, Gyeongsan, Republic of Korea, Kim, Y.W., College of Medicine, Yeungnam University, Daegu, Republic of Korea, Shin, D.H., College of Medicine, Yeungnam University, Daegu, Republic of Korea, Kim, J.Y., College of Medicine, Yeungnam University, Daegu, Republic of Korea, Kim, K.J., Tae Kyeung College, Gyeongsan, Republic of Korea, Lee, K.H., Eulji University, Daejeon, Republic of Korea, Cheon, Y.P., Sungshin Women's University, Seoul, Republic of Korea, Chun, T.H., Korea University, Seoul, Republic of Korea, Choi, I.H., Yeungnam University, Gyeongsan, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Daejeon Taylor & Francis Group 01.12.2012; Taylor & Francis Ltd; 한국통합생물학회
• Subjects: Animals; Carbon dioxide; COLAGENO; COLLAGEN; COLLAGENE; Epidermis; Hormones; Medical treatment; Periodontal diseases; PTSE; skin injuries; Wound healing; 생물학
• ISSN: 1976-8354; 2151-2485
• DOI: 10.1080/19768354.2012.726645

Sex hormones have long been considered to play an important role in bone turnover rate, periodontal diseases, and wound healing. We have studied the effect of porcine testis steroid extract (PTSE), an extract of porcine testes, which holds a good ratio of 19-nortestosterone (nandrolone), testosterone, androstenedione, 17β-estradiol, and estrone, on the healing rate of a standardized full-thickness linear wound on the back of the rat. Skin punch or carbon dioxide (CO₂) laser methods were used to create the deep skin injury in two groups of animals. The animals were treated with the PTSE cream, control cream and Vaseline (control) to find out the effect in re-epithelialization, contraction, and formation of granulation and scar tissues. Histological examination after 21 days showed 100, 87.4, and 80.5% recovery of epidermis, dermis, and hypodermis, respectively in the PTSE-treated animals. Similarly, on the 15th day of treatment, complete healing of intact skin was observed in the PTSE cream-treated animals among the laser radiation group. Even though the beginning of re-epithelialization phase and completion of serum crust formation was also observed in the base cream- and Vaseline-treated animals respectively, the complete healing cycle was observed only in the PTSE-treated group. The white blood cell count in the PTSE-treated group showed that PTSE cream is nontoxic to animals.