Decay-accelerating Factor (DAF), Complement Receptor 1 (CR1), and Factor H Dissociate the Complement AP C3 Convertase (C3bBb) via Sites on the Type A Domain of Bb

• Published in: The Journal of biological chemistry Vol. 277; no. 2; pp. 1107 - 1112
• Main Authors: Hourcade, Dennis E., Mitchell, Lynne, Kuttner-Kondo, Lisa A., Atkinson, John P., Medof, M. Edward
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 11.01.2002
• Subjects: CD55 Antigens - metabolism; Cell Line; complement AP C3 convertase; Complement C3-C5 Convertases - chemistry; Complement C3-C5 Convertases - genetics; Complement C3-C5 Convertases - metabolism; complement component C3b; complement factor H; Complement Factor H - metabolism; complement receptor 1; Culture Media, Serum-Free; decay accelerating factor; factor H; Humans; Models, Molecular; Multienzyme Complexes; Mutagenesis, Site-Directed; Protein Structure, Tertiary; Receptors, Complement - metabolism
• ISSN: 0021-9258; 1067-8816; 1083-351X; 1083-351X
• DOI: 10.1074/jbc.M109322200

The AP C3 convertase, C3bBb(Mg 2+ ), is subject to irreversible dissociation (decay acceleration) by three proteins: DAF, CR1, and factor H. We have begun to map the factor B (fB) sites critical to these interactions. We generated a panel of fB mutations, focusing on the type A domain because it carries divalent cation and C3b-binding elements. C3bBb complexes were assembled with the mutants and subjected to decay acceleration. Two critical fB sites were identified with a structural model. 1) Several mutations centered at adjacent alpha helices 4 and 5 (Gln-335, Tyr-338, Ser-339, Asp-382) caused substantial resistance to DAF and CR1-mediated decay acceleration but not factor H. 2) Several mutations centered at the α 1 helix and adjoining loops (especially D254G) caused resistance to decay acceleration mediated by all three regulators and also increased C3b-binding affinity and C3bBb stability. In the simplest interpretation of these results, DAF and CR1 directly interact with C3bBb at α 4/5; factor H likely interacts at some other location, possibly on the C3b subunit. Mutations at the C3b·Bb interface interfere with the normal dissociation of C3b from Bb, whether it is spontaneous or promoted by DAF, CR1, or factor H.