Tandem mass spectrometry approaches for recognition of isomeric compounds mixtures

• Published in: Mass spectrometry reviews Vol. 42; no. 4; pp. 1244 - 1260
• Main Authors: Crotti, Sara, Menicatti, Marta, Pallecchi, Marco, Bartolucci, Gianluca
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.07.2023
• Subjects: Chemical modification; ion‐molecule interaction; Isomers; kinetic method; LEDA; Mass spectroscopy; MS/MS; Stereoisomers; survival yield; kinetic method; ion-molecule interaction; isomers; MS/MS; LEDA; survival yield
• ISSN: 0277-7037; 1098-2787; 1098-2787
• DOI: 10.1002/mas.21757

The present review aims to collect the published literature pertaining the recognition of isobaric compounds (isomers or stereoisomers) using the features of tandem mass spectrometry (MS) experiments without any chromatographic separation or chemical modification (derivatization or isotopic enrichment) of the analytes. MS/MS methods possess high selectivity, wide dynamic range and high throughput capabilities. Generally, tandem MS has limited capability for distinguishing isomers that fragment similarly. However, some MS/MS methods have been developed and positively applied to isomers discrimination. Among the literature on this topic, the applications that fit on the review subject can be summarized as follow: (1) chiral discrimination by the kinetic method, (2) the use energy‐resolved tandem mass spectra and the survival yield (SY) representation, (3) the kinetics evaluation of the ion‐molecule interaction and (4) the postprocessing mathematical algorithm to resolve the isomers in MS/MS signal.