Molecular analysis of soyasaponin biosynthetic genes in two soybean (Glycine max L. Merr.) cultivars

• Published in: Plant biotechnology reports Vol. 15; no. 1; pp. 117 - 124
• Main Authors: Yun, Young Jae, Lee, HanGyeol, Yoo, Dong Jin, Yang, Ji Yeong, Woo, So-Yeun, Seo, Woo Duck, Kim, Young-Cheon, Lee, Jeong Hwan
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.02.2021; Springer Nature B.V; 한국식물생명공학회
• Subjects: abiotic stress; Agriculture; biochemical pathways; Biomedical and Life Sciences; Biosynthesis; Biotechnology; Cell Biology; Cultivars; Gene expression; Genes; Glycine max; Life Sciences; Plant Biochemistry; Plant Sciences; Short Communication; Soyasaponin; Soybeans; 농학; Socheongja; Soyasaponin; Soybean; Abiotic stress; Haepum
• ISSN: 1863-5466; 1863-5474
• DOI: 10.1007/s11816-021-00661-w

Here, we identified sequence variations of CYP72A69 and UGT73F4 genes from the Sojeongja and Haepum cultivars, and revealed that accumulation patterns of soyasaponin Aa and Ab were different in the Sojeongja and Haepum cultivars. Several genes related in soyasaponin biosynthetic pathway showed significant changes in their expression patterns in two soybean cultivars and soyasaponin contents were also altered under several abiotic stress conditions, albeit no significant correlations between soyasaponin contents and biosynthetic gene expression were observed. We also demonstrated that UGT73F4 gene could be responsible for biosynthesis of soyasaponin Aa in the Socheongja by investigating gene expression and soyasaponin accumulations. Our results provide a molecular basis to improve the commercial properties of soyasaponin in Korean domestic soybean cultivars.