Surface-modified nanofibrous biomaterial bridge for the enhancement and control of neurite outgrowth

Biomaterial bridges constructed from electrospun fibers offer a promising alternative to traditional nerve tissue regeneration substrates. Aligned and unaligned polycaprolactone (PCL) electrospun fibers were prepared and functionalized with the extracellular matrix proteins collagen and laminin usin...

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Published inBiointerphases Vol. 5; no. 4; pp. 149 - 158
Main Authors Zander, Nicole E., Orlicki, Joshua A., Rawlett, Adam M., Beebe, Thomas P.
Format Journal Article
LanguageEnglish
Published United States 01.12.2010
Subjects
Adsorption
Analysis of Variance
Animals
Biocompatible Materials - chemistry
Cell Differentiation
Cell Proliferation
Cell Survival
Collagen - metabolism
Electrochemical Techniques
Laminin - metabolism
Microscopy, Confocal
Microscopy, Electron, Scanning
Nanofibers - chemistry
Nanofibers - ultrastructure
Neurites - metabolism
Neurites - physiology
PC12 Cells
Rats
Tissue Culture Techniques - methods
Tissue Scaffolds
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ISSN1934-8630
1559-4106
1559-4106
DOI10.1116/1.3526140

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Summary:Biomaterial bridges constructed from electrospun fibers offer a promising alternative to traditional nerve tissue regeneration substrates. Aligned and unaligned polycaprolactone (PCL) electrospun fibers were prepared and functionalized with the extracellular matrix proteins collagen and laminin using covalent and physical adsorption attachment chemistries. The effect of the protein modified and native PCL nanofiber scaffolds on cell proliferation, neurite outgrowth rate, and orientation was examined with neuronlike PC12 cells. All protein modified scaffolds showed enhanced cellular adhesion and neurite outgrowth compared to unmodified PCL scaffolds. Neurite orientation was found to be in near perfect alignment with the fiber axis for cells grown on aligned fibers, with difference angles of less than 7o from the fiber axis, regardless of the surface chemistry. The bioavailability of PCL fibers with covalently attached laminin was found to be identical to that of PCL fibers with physically adsorbed laminin, indicating that the covalent chemistry did not change the protein conformation into a less active form and the covalent attachment of protein is a suitable method for enhancing the biocompatibility of tissue engineering scaffolds. a) Electronic mail: nicole.zander@arl.army.mil
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ISSN:1934-8630
1559-4106
1559-4106
DOI:10.1116/1.3526140