Effects of different light conditions on repair of UV-B-induced damage in carpospores of Chondrus ocellatus Holm

• Published in: Chinese journal of oceanology and limnology Vol. 33; no. 3; pp. 664 - 678
• Main Author: 鞠青 肖慧 王悠 唐学玺
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer-Verlag 01.05.2015; Science Press; Springer Nature B.V
• Subjects: Amino acids; Biology; blue light; branches; Carpospores; Chlorophyll; Chlorophylls; Chondrus; Chondrus ocellatus; Color; Cyclobutane; Cyclobutane pyrimidine dimers; Damage; Darkness; Deoxyribonucleic acid; Diameters; DNA; DNA repair; Earth and Environmental Science; Earth Sciences; growth and development; Growth rate; Laboratory experimentation; Light; Mycosporine-like amino acids; Oceanography; Photolyase; Photosynthesis; Photosynthetic pigments; Photosynthetically active radiation; phycoerythrin; pigments; Pyrimidines; Radiation; Radiation damage; red light; remediation; Rhodophyta; Shinorine; Ultraviolet radiation; UV-B辐射; 修复效果; 光照条件; 损伤; 果孢子; 环丁烷嘧啶二聚体; 生长速率; 红鱼; repair; blue light; Holm; red light; UV-B radiation
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-015-4261-0

We evaluated the effects of ultraviolet-B （UV-B） radiation and different light conditions on the repair of UV-B-induced damage in carpospores of Chondrus ocellatus Holm （Rhodophyta） in laboratory experiments. Carpospores were treated daily with different doses of UV-B radiation for 48 days, when vertical branches had formed in all treatments; after each daily treatment, the carpospores were subjected to photosynthetically active radiation （PAR）, darkness, red light, or blue light during a 2-h repair stage. Carpospore diameters were measured every 4 days. We measured the growth and cellular contents of cyclobutane pyrimidine dimers （CPDs）, chlorophyll a, phycoerythrin, and UV-B-absorbing mycosporine-like amino acids （MAAs） in carpospores on Day 48. Low doses of UV-B radiation （36 and 72 J/m2） accelerated the growth of C. ocellatus. However, as the amount of UV-B radiation increased, the growth rate decreased and morphological changes occurred. UV-B radiation significant damaged DNA and photosynthetic pigments and induced three kind of MAAs, palythine, asterina-330, and shinorine. PAR conditions were best for repairing UV-B-induced damage. Darkness promoted the activity of the DNA dark- repair mechanism. Red light enhanced phycoerythrin synthesis but inhibited light repair of DNA. Although blue light, increased the activity of DNA photolyase, greatly improving remediation efficiency, the growth and development of C. ocellatus earpospores were slower than in other light treatments.