Screening of marine fungus from Nanji Island and activity of their metabolites against pathogenic Vibrio from Pseudosciaena crocea

• Published in: Chinese journal of oceanology and limnology Vol. 30; no. 5; pp. 746 - 756
• Main Author: 赵淑江 李书平 刘辉辉 赵倩 王杰优 闫茂仓
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer-Verlag 01.09.2012; SP Science Press; Springer Nature B.V
• Subjects: agar; Antibiotics; Antimicrobial agents; Arthrinium; Bacteria; Biology; Butanol; China; Earth and Environmental Science; Earth Sciences; Fungi; Larimichthys crocea; Marine; Marine biology; marine fungi; Metabolites; Nucleotide sequence; Oceanography; Pathogens; polymerase chain reaction; Pseudosciaena crocea; ribosomal DNA; Screening; Sediment samplers; Sediment samples; Sediments; Stability; Strains; Strains (organisms); Thermal stability; Vibrio; 代谢产物; 南麂岛; 大黄鱼; 海洋沉积物样品; 海洋真菌; 真菌菌株; 筛选; 致病性弧菌; China; Zhejiang China; ISEW, China, People's Rep., Zhejiang Prov; ISEW, China, People's Rep., Zhejiang Prov., Nanji I; ITS-5.8S rDNA; Nanji Island; activity; marine fungi; stability
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-012-2003-0

Seventy-eight marine fungal strains were isolated from sediment samples collected off the coast of Nanji Island, Wenzhou, Zhejiang Province, China. Antibacterial screening using the agar disc method showed that 19 of the isolated strains could inhibit at least one pathogenic Vibrio from Pseudosciaena crocea. Subsequent screening confirmed that nine strains produced antibacterial metabolites that had activity against one or several types of pathogenic Vibrio. Strain NJ0104 had the widest antimicrobial spectrum and strong activity, particularly against Fibrio parahaemolyticus-MM0810072. A preliminary study of N J0104 antibacterial metabolites demonstrated that they had thermal stability up to 80℃, ultraviolet stability up to 40 min and pH stability between 4.0-7.0. In addition, the antibacterial metabolites were readily soluble in butanol. To identify the specific strain, the ITS-5.8S rDNA regions of NJ0104 were PCR amplified and sequenced. Based on the combination of phenotypic and genotypic data, the strain was identified as Arthrinium sp.