Electrochemical immunosensor based on AuNPs/Zn/Ni-ZIF-8-800@graphene for rapid detection of aflatoxin B1 in peanut oil

• Published in: Analytical biochemistry Vol. 650; p. 114710
• Main Authors: Wang, Na, Liu, Qingqing, Hu, Xiaofei, Wang, Fan, Hu, Mei, Yu, Qiuying, Zhang, Gaiping
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2022
• Subjects: Aflatoxin B1; antibodies; AuNPs/Zn/Ni-ZIF-8-800@graphene; chitosan; detection limit; Electrochemical immunosensor; electrochemistry; food safety; Fourier transform infrared spectroscopy; glassy carbon electrode; graphene; immunosensors; nanogold; Peanut oil; Rapid detection; rapid methods; raw materials; voltammetry; X-ray diffraction; X-ray photoelectron spectroscopy; Aflatoxin B1; Electrochemical immunosensor; AuNPs/Zn/Ni-ZIF-8-800@graphene; Rapid detection; Peanut oil
• ISSN: 0003-2697; 1096-0309; 1096-0309
• DOI: 10.1016/j.ab.2022.114710

Peanut oil is a basic food raw material in life. However, aflatoxin contamination in peanut oil is considered to be one of the most serious food safety problems in the world. Based on AuNPs/Zn/Ni-ZIF-8-800@graphene composite, a simple, efficient and sensitive electrochemical immunosensor was developed to detect aflatoxin B1 (AFB1) in peanut oil. The bare glassy carbon electrode was modified by graphene, bimetallic organic framework material (Zn/Ni-ZIF-8-800), chitosan and gold nanoparticles. The electrochemical immunosensor was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FT-IR) and cyclic voltammetry (CV), and the electrochemical signal changes after antibody and AFB1 binding were investigated in detail. Under the optimal conditions, the linear range of the electrochemical immunosensor was 0.18–100 ng/mL, and the detection limit was 0.18 ng/mL. In addition, the prepared sensor has high selectivity and long-term stability, which lays a foundation for the simple, rapid and sensitive detection of AFB1 in peanut oil. [Display omitted] •An electrochemical sensor based on AuNPs/Zn/Ni-ZIF-8-800@graphene for the detection of AFB1 in peanut oil has not been reported yet.•The prepared sensor has high selectivity and long-term stability, which lays a foundation for the simple, rapid and sensitive detection of AFB1 in peanut oil, and the detection limit of AFB1 in peanut oil is 0.18 ng/ml, which is lower than the limit standard of Chinese and EU.