Placental Polycyclic Aromatic Hydrocarbon (PAH) Levels Are Associated with Spontaneous Preterm Birth

• Published in: International journal of molecular sciences Vol. 26; no. 17; p. 8179
• Main Authors: Hummel, Gwendolynn, Banerjee, Sohini, Putluri, Vasanta, Malick, Inaara, Johnson, Grace, Kamal, Abu Hena Mostafa, Ambati, Chandra Shekar R., Putluri, Nagireddy, Showalter, Lori, Shope, Cynthia D., Hagan, Joseph, Aagaard, Kjersti M., Moorthy, Bhagavatula, Suter, Melissa A.
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.09.2025
• Subjects: Adult; Benzo(a)pyrene - analysis; Birth weight; Ethnicity; Female; Females; Gestational Age; Humans; Hydrocarbons; Hypotheses; Infant, Newborn; Infants (Premature); Male; Males; Mass spectrometry; Placenta; Placenta - chemistry; Placenta - metabolism; Polycyclic Aromatic Hydrocarbons - analysis; Polycyclic Aromatic Hydrocarbons - metabolism; Pregnancy; Premature birth; Premature Birth - etiology; Premature Birth - metabolism; Risk factors; United States; Texas; United States > US; environmental health; Superfund; pregnancy
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms26178179

While the cause of preterm birth (PTB) (i.e., delivery before 37 weeks of gestation) is likely multifactorial, ambient exposure to environmental chemicals has been postulated to play a role in its etiology. Our prior studies of exposure to polycyclic aromatic hydrocarbons (PAHs) in pregnancy have shown an increased level of placental PAH-induced bulky DNA adducts with increasing levels of PAH exposures. In this investigation, we hypothesized that higher levels of placental PAHs would be associated with an increased risk of PTB. Using gas chromatography and mass spectrometry (GC-MS/MS), we measured levels of benzo(a)pyrene (BaP), benzo(b)fluoranthene (BbF) and dibenz(a,h)anthracene (DBA) from n = 323 subjects. We found higher levels of BbF in placentae collected from preterm compared with term deliveries (mean 100.3 vs. 84.14 ng/mL, p = 0.038). Placental BbF levels negatively correlated with gestational age at delivery (rs = −0.171, p = 0.002) and placental DBA levels were higher in placentae from spontaneous PTBs compared to those that were medically indicated (mean 743.7 vs. 599.9 ng/mL, p = 0.049), suggesting a potentially causal role in spontaneous preterm birth. Lastly, we analyzed placental levels of each PAH in male (n = 164) and female (n = 159) gestations and found that levels of BaP are significantly higher in males (mean 204.4 vs. 169.9 ng/mL, p = 0.049). These studies show a potential causal role of PAH exposure in the etiology of spontaneous preterm birth.