Retinal organoids: a window into human retinal development

• Published in: Development (Cambridge) Vol. 147; no. 24
• Main Authors: O'Hara-Wright, Michelle, Gonzalez-Cordero, Anai
• Format: Journal Article
• Language: English
• Published: England The Company of Biologists Ltd 24.12.2020
• Subjects: Animals; Brain - growth & development; Cell Differentiation - genetics; Corticomedial Nuclear Complex - growth & development; Human Embryonic Stem Cells - cytology; Humans; Induced Pluripotent Stem Cells - cytology; Organogenesis - genetics; Organoids - growth & development; Photoreceptor Cells - cytology; Retina - growth & development; Review; Retinal organoids; Human development; Stem cells
• ISSN: 0950-1991; 1477-9129; 1477-9129
• DOI: 10.1242/dev.189746

Retinal development and maturation are orchestrated by a series of interacting signalling networks that drive the morphogenetic transformation of the anterior developing brain. Studies in model organisms continue to elucidate these complex series of events. However, the human retina shows many differences from that of other organisms and the investigation of human eye development now benefits from stem cell-derived organoids. Retinal differentiation methods have progressed from simple 2D adherent cultures to self-organising micro-physiological systems. As models of development, these have collectively offered new insights into the previously unexplored early development of the human retina and informed our knowledge of the key cell fate decisions that govern the specification of light-sensitive photoreceptors. Although the developmental trajectories of other retinal cell types remain more elusive, the collation of omics datasets, combined with advanced culture methodology, will enable modelling of the intricate process of human retinogenesis and retinal disease in vitro.