Establishment of Hamster- and Human-PRNP Transgenic Mice

• Published in: Biomedical and environmental sciences Vol. 24; no. 6; pp. 608 - 616
• Main Authors: GONG, Han Shi, TIAN, Chan, ZHANG, Bao Yun, WANG, Zhao Yun, XIE, Wu Ling, JING, Yuan Yuan, GAO, Chen, JIANG, Hui Ying, SHI, Qi, LIU, Yong, DONG, Xiao Ping
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.12.2011; State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China%State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China%School of Medicine, Xi'an Jiao Tong University, Xi'an 710061, Shaanxi, China; School of Medicine, Xi'an Jiao Tong University, Xi'an 710061, Shaanxi, China
• Subjects: Animals; Blotting, Western; blot分析; brain; Copy number; Cricetinae; Disease Models, Animal; DNA - genetics; hamsters; Humans; Immunohistochemistry; messenger RNA; Mice; Mice, Inbred C57BL; Mice, Transgenic; Organ Specificity; Plasmids; prion diseases; Prion Diseases - genetics; Prion Proteins; prions; Prions - genetics; PRNP; PrP; quantitative polymerase chain reaction; Real-Time Polymerase Chain Reaction; reverse transcriptase polymerase chain reaction; RT-PCR技术; staining; transcription (genetics); Transcription, Genetic; transgenic animals; Transgenic mice; translation (genetics); Western blotting; 人类; 传染性海绵状脑病; 实时RT-PCR; 朊病毒; 蛋白水平; 转基因小鼠; PRNP; Copy number; Transgenic mice; PrP
• ISSN: 0895-3988; 2214-0190
• DOI: 10.3967/0895-3988.2011.06.004

Objective To create transgenic mice expressing hamster- and human-PRNP as a model tor understanding the physiological function and pathology of prion protein （PrP）, as well as the mechanism of cross-species transmission of transmissible spongiform encephalopathies （TSEs）. Methods Hamster and human-PRNP transgenic mice were established by conventional methods. The copy number of integrated PRNP in various mouse lines was mapped by real-time PCR. PRNP mRNA and protein levels were determined by semi-quantitative RT-PCR, real-time RT-PCR, and western blot analysis. Histological analyses of transgenic mice were performed by hematoxylin and eosin （H ＆ E） staining and immunohistochemical （IHC） methods. Results Integrated PRNP copy number in various mouse lines was 53 （Tg-haPrP1）, 18 （Tg-huPrP1）, 3 （Tg-huPrP2）, and 16 （Tg-huPrP5）, respectively. Exogenous PrPs were expressed at both the transcriptional and translational level. Histological assays did not detect any abnormalities in brain or other organs. Conclusion We have established one hamster-PRNP transgenic mouse line and three human-PRNP transgenic mouse lines. These four transgenic mouse lines provide ideal models for additional research.