Fecal Microbial Dysbiosis Is Associated with Colorectal Cancer Risk in a Korean Population

• Published in: Cancer research and treatment Vol. 57; no. 1; pp. 198 - 211
• Main Authors: Kim, Jeongseon, Gunathilake, Madhawa, Yeo, Hyun Yang, Oh, Jae Hwan, Kim, Byung Chang, Han, Nayoung, Kim, Bun, Pyun, Hyojin, Lim, Mi Young, Nam, Young-Do, Chang, Hee Jin
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Cancer Association 01.01.2025; 대한암학회
• Subjects: Adult; Aged; Case-Control Studies; Colorectal Neoplasms - epidemiology; Colorectal Neoplasms - etiology; Colorectal Neoplasms - microbiology; Dysbiosis - complications; Dysbiosis - epidemiology; Dysbiosis - microbiology; Feces - microbiology; Female; Gastrointestinal Microbiome; Humans; Male; Middle Aged; Original; Republic of Korea - epidemiology; Risk Factors; RNA, Ribosomal, 16S - genetics; 의학일반; Republic of Korea; Microbial dysbiosis; 16S rRNA gene sequencing; Fecal microbiome; Colorectal neoplasms; Microbial metabolic pathways
• ISSN: 1598-2998; 2005-9256; 2005-9256
• DOI: 10.4143/crt.2024.382

Purpose The association between the fecal microbiota and colorectal cancer (CRC) risk has been suggested in epidemiologic studies. However, data from large-scale population-based studies are lacking.Materials and Methods In this case-control study, we recruited 283 CRC patients from the Center for Colorectal Cancer, National Cancer Center Hospital, Korea to perform 16S rRNA gene sequencing of fecal samples. A total of 283 age- and sex-matched healthy participants were selected from 890 cohort of healthy Koreans that are publicly available (PRJEB33905). The microbial dysbiosis index (MDI) was calculated based on the differentially abundant species. The association between MDI and CRC risk was observed using conditional logistic regression. Sparse Canonical Correlation Analysis was performed to integrate species data with microbial pathways obtained by PICRUSt2.Results There is a significant divergence of the microbial composition between CRC patients and controls (permutational multivariate analysis of variance p=0.001). Those who were in third tertile of the MDI showed a significantly increased risk of CRC in the total population (odds ratio [OR], 6.93; 95% confidence interval [CI], 3.98 to 12.06; p-trend < 0.001) compared to those in the lowest tertile. Similar results were found for men (OR, 6.28; 95% CI, 3.04 to 12.98; p-trend < 0.001) and women (OR, 7.39; 95% CI, 3.10 to 17.63; p-trend < 0.001). Bacteroides coprocola and Bacteroides plebeius species and 12 metabolic pathways were interrelated in healthy controls that explain 91% covariation across samples.Conclusion Dysbiosis in the fecal microbiota may be associated with an increased risk of CRC. Due to the potentially modifiable nature of the gut microbiota, our findings may have implications for CRC prevention among Koreans.