Lactobacillus johnsonii JERA01 upregulates the production of Th1 cytokines and modulates dendritic cells-mediated immune response

• Published in: The Korean journal of physiology & pharmacology Vol. 29; no. 3; pp. 271 - 281
• Main Authors: Kim, Si-Yeon, Joo, Hong-Gu
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Physiological Society and The Korean Society of Pharmacology 01.05.2025; 대한약리학회
• Subjects: Original; 약리학; T-lymphocytes; Cytokines; Dendritic cells; Immunity
• ISSN: 1226-4512; 2093-3827
• DOI: 10.4196/kjpp.24.205

Lactic acid bacteria are known to have various effects on the immune system. The type and extent of the effect differ, depending on the type of lactic acid bacteria. This study aimed to investigate the effects of Lactobacillus johnsonii bacterin on mouse-derived immune cells. Treating splenocytes with bacterin slightly increased the metabolic activity. Additionally, the expression of the activation marker CD25 and production of the Th1-type inflammatory cytokine interferon (IFN)- gamma increased. We confirmed that the increase in IFN-gamma production due to stimulation was mainly due to T and B cells among splenocytes. Treating dendritic cells (DCs) with bacterin at concentrations of 10 and 10 cfu/ ml significantly increased tumor necrosis factor-alpha, a pro-inflammatory cytokine, and interleukin-12, a cell-mediated immunity cytokine. Additionally, the expression of surface markers increased. Allogeneic mixed lymphocyte reactions showed that reduced the antigen-presenting ability of DCs. In cocultures of DCs and splenocytes, decreased cellular metabolic activity and increased cell death. upregulated the expression of programmed death ligand 1 on DCs. The findings of this study indicate that bacterin has immunomodulatory and immunostimulatory effects. While increased the expression of cytokines and surface markers of immune cells, it modulated DC-mediated immune response. Further studies are needed to determine the effects of bacterin on DCs and related immune cells.