Thymic function recovery after unrelated donor cord blood or T-cell depleted HLA-haploidentical stem cell transplantation correlates with leukemia relapse

• Published in: Frontiers in immunology Vol. 4; p. 54
• Main Authors: Clave, Emmanuel, Lisini, Daniela, Douay, Corinne, Giorgiani, Giovanna, Busson, Marc, Zecca, Marco, Moretta, Francesca, Acquafredda, Gloria, Brescia, Letizia P., Locatelli, Franco, Toubert, Antoine
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 2013
• Subjects: HSCT; Immunology; Leukemia; relapse; T cells; Thymic function; leukemia; T cells; relapse; HSCT; thymic function
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2013.00054

Use of alternative donors/sources of hematopoietic stem cells (HSC), such as cord blood (CB) or HLA-haploidentical (Haplo)-related donors, is associated with a significant delay in immune reconstitution after transplantation. Long-term T-cell immune reconstitution largely relies on the generation of new T cells in the recipient thymus, which can be evaluated through signal joint (sj) and beta T-cell-Receptor Excision Circles (TREC) quantification. We studied two groups of 33 and 24 children receiving, respectively, HSC Transplantation (HSCT) from an HLA-haploidentical family donor or an unrelated CB donor, for both malignant (46) and non-malignant disorders (11). Relative and absolute sj and beta-TREC values indicated comparable thymic function reconstitution at 3 and 6 months after the allograft in both groups. Compared to children with non-malignant disorders, those with hematological malignancies had significantly lower pre-transplantation TREC counts. Patients who relapsed after HSCT had a significantly less efficient thymic function both before and 6 months after HSCT with especially low beta-TREC values, this finding suggesting an impact of early intra-thymic T-cell differentiation on the occurrence of leukemia relapse.