A family of tubular pili from harmful algal bloom forming cyanobacterium Microcystis aeruginosa

• Published in: Nature communications Vol. 16; no. 1; pp. 8082 - 13
• Main Authors: Ricca, John G., Petersen, Holly A., Grosvirt-Dramen, Adam, Mayali, Xavier, Naylon, Sarah H., Duersch, Bobby G., Dufresne, Craig P., Weber, Peter K., Sonani, Ravi R., Prevelige, Peter E., Hochbaum, Allon I., Merk, Vivian, Louda, J. W., Wang, Fengbin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 29.08.2025; Nature Publishing Group; Nature Portfolio
• Subjects: 101/28; 14/63; 631/535/1258/1259; 631/57/2272/2276; 704/829/826; Algae; Algal blooms; Bacteria; Bacterial Proteins - metabolism; Buoyancy; Cell morphology; Colonies; Cryoelectron Microscopy; Cyanobacteria; Drag; Electron microscopes; Enrichment; Eutrophication; Extracellular matrix; Fimbriae Proteins - chemistry; Fimbriae Proteins - metabolism; Fimbriae Proteins - ultrastructure; Fimbriae, Bacterial - chemistry; Fimbriae, Bacterial - metabolism; Fimbriae, Bacterial - ultrastructure; Glycosylation; Harmful Algal Bloom; Humanities and Social Sciences; Iron; Microcystins; Microcystins - metabolism; Microcystis; Microcystis - genetics; Microcystis - metabolism; Microcystis - ultrastructure; Microcystis aeruginosa; Monosaccharides; Morphology; multidisciplinary; Pili; Pilin; Prokaryotes; Protein folding; Proteins; Scanning electron microscopy; Science; Science (multidisciplinary); Sediments; Structural analysis; Structure-function relationships; Toxins; Water quality
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-025-63379-1

Cyanobacteria are vital photosynthetic prokaryotes, but some form harmful algal blooms (cyanoHABs) that disrupt ecosystems and produce toxins. The mechanisms by which these blooms form have yet to be fully understood, particularly the role of extracellular components. Here, we present a 2.4 Å cryo-EM structure of a pilus, termed the cyanobacterial tubular (CT) pilus, found in the cyanoHAB-forming Microcystis aeruginosa . The pilin exhibits a unique protein fold, forming a tubular pilus structure with tight, double-layer anti-parallel β-sheet interactions. We show that CT pili are essential for buoyancy by facilitating the formation of micro-colonies, which increases drag force and prevents sinking. The CT pilus surface is heavily glycosylated with ten monosaccharide modifications per pilin. Furthermore, CT pili can enrich microcystin, potentially enhancing cellular resilience, and co-localize with iron-enriched extracellular matrix components. Thus, we propose that this pilus plays an important role in the proliferation of cyanoHABs. This just discovered pilus family appears to be widely distributed across several cyanobacterial orders. Our structural and functional characterization of CT pili provide insights into cyanobacterial cell morphology, physiology, and toxin interactions, and identify potential targets for disrupting bloom formation. Ricca et al discover a new family of tubular pili in Microcystis aeruginosa, a harmful algal bloom-forming cyanobacterium. These pili are crucial for buoyancy by forming cell micro-colonies, which increases drag and prevents sinking. The pili also enrich microcystin and co-localize with iron-enriched extracellular matrix components, suggesting a vital role in bloom proliferation.