Bacterial diversity in surface water of the Yellow Sea during and after a green alga tide in 2008

• Published in: Chinese journal of oceanology and limnology Vol. 29; no. 6; pp. 1147 - 1154
• Main Author: 郭丛 李富超 姜鹏 刘兆普 秦松
• Format: Journal Article
• Language: English
• Published: Heidelberg SP Science Press 01.11.2011; Springer Nature B.V
• Subjects: Actinobacteria; Algae; Alphaproteobacteria; Aquatic plants; Bacteria; Bacteroidetes; Biology; Chemical analysis; Clones; Cyanobacteria; Earth and Environmental Science; Earth Sciences; Enteromorpha prolifera; Gammaproteobacteria; Green tides; Marine; Marine biology; Marine ecosystems; Microbiomes; Microorganisms; Oceanography; Planctomycetes; Polymorphism; Proteobacteria; Restriction fragment length polymorphism; rRNA 16S; Seawater; Surface water; Taxonomy; Ulva; Verrucomicrobia; Water analysis; 克隆测序; 地表水; 微生物群落; 生物群落多样性; 细菌多样性; 藻类; 限制性片段长度多态性; 黄海; Yellow Sea; INW, Huanghai Sea; China, People's Rep; operational taxonomic unit (OTU); restriction fragment length polymorphism (RFLP); 16S rRNA gene
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-011-0264-7

From May to August 2008, a large ＂green tide＂, consisting of the alga Ulva （Enteromorpha） prolifera, occurred in the Yellow Sea, China, affecting the local marine ecosystem and human activities. We investigated the influence of the green tide on the microbial community in the surface seawater, at four sites from July to August 2008, using bacterial 16S rRNA gene clone libraries. We sequenced 228 clones of unique patterns identified by restriction fragment length polymorphism （RFLP） techniques. The results show that 228 sequenced clones fell into six bacterial phyla： Proteobacteria, Bacteroidetes, Cyanobacteria, Verrucomicrobia, Actinobacteria, and Planctomycetes. Alphaproteobacteria （33%）, Gammaproteobacteria （25%）, Bacteroidetes （23%） and Cyanobacteria （9%） dominated the assemblage. Comparison between samples collected in July （during the tide） and those collected in August （after the tide） showed that, in the microbial community, diversities of Alphaproteobacteria and Cyanobacteria increased after the tide, while those of Gammaproteobacteria and Bacteroidetes decreased. These results indicate that the green tide influenced the growth of some bacteria, and provide information for further studies on the interactions and relationships between U. prolifera and the bacterial community. This study suggests that microbial community analysis is a good approach to monitoring green tides.