Determination of Betamethasone and Dexamethasone in Human Urine and Serum by MEKC After an Experimental Design

• Published in: Chromatographia Vol. 68; no. 3-4; pp. 287 - 293
• Main Authors: Song, Lihua, Bai, Jianguo, Zhou, Weihong
• Format: Journal Article
• Language: English
• Published: Wiesbaden Wiesbaden : Vieweg Verlag 01.08.2008; Vieweg Verlag; Springer
• Subjects: Analysis; Analytical Chemistry; betamethasone; Biological and medical sciences; blood serum; borax; Chemistry; Chemistry and Materials Science; Chromatography; correlation; dexamethasone; experimental design; Full Short Communication; General pharmacology; humans; Laboratory Medicine; Medical sciences; micellar electrokinetic capillary chromatography; Pharmacology. Drug treatments; Pharmacy; Proteomics; sodium; sodium dodecyl sulfate; urine; Human urine and serum; Experimental design; Betamethasone and dexamethasone; Micellar electrokinetic capillary chromatography; Human; Steroid; Urine; Validation; Corticosteroid; Biological fluid; Chemical analysis; Dexamethasone; Steroid hormone; Antiinflammatory agent; Micellar electrokinetic capillary chromatography,Experimental design,Human urine and serum,Betamethasone and dexamethasone; Optimization; Blood; Ultraviolet detector; Organic fluorine compounds; Betamethasone; Factorial design; Blood serum; Chemometrics; Quantitative analysis
• ISSN: 0009-5893; 1612-1112
• DOI: 10.1365/s10337-008-0692-5

A simple and reliable micellar electrokinetic capillary chromatography method has been presented for the simultaneous determination of betamethasone (BM) and its epimer dexamethasone (DM) in human urine and serum. A three level full factorial experimental design was employed to search for the optimum conditions. Rapid and baseline separation of BM and DM was obtained within 7 min with the optimum conditions of 30 mM borax buffer, 30 mM sodium dodecyl sulfate at pH 10.0, separation voltage at 18 kV, injection time 15 s at a height of 10 cm, using sodium sorbate as internal standard. The proposed method was validated with respect to stability, precision, linearity and accuracy. Good relationship between peak area ratio and analyte concentration was linear over 30-1,000 μg mL⁻¹ for BM and DM with correlation coefficients >=0.9993. Relative standard deviations of the method were all less than 4.50% in the intra-day and inter-day analysis. The developed method was applied to assay spiked human urine and serum samples containing both compounds with recoveries in the range of 97.5-100.5%.