Photochemical Fluorimetric Determination of Cephradine

• Published in: Analytical letters Vol. 31; no. 3; pp. 439 - 450
• Main Authors: Cai, Wei-Ping, Ouyang, Yao-Guo, Lin, Xue-Yuan, Xu, Jin-Gou
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Taylor & Francis Group 01.02.1998; Taylor & Francis
• Subjects: Analysis; Biological and medical sciences; Cephradine; Fluorimetry; General pharmacology; Medical sciences; Pharmacology. Drug treatments; Photochemical method; Trace analysis; Urine; Sulfur nitrogen heterocycle; Chemical analysis; Cefradine; Photochemical method; Fluorescence spectrometry; Antibiotic; Cephalosporin derivatives; Near ultraviolet radiation; Bicyclic compound; Reaction mechanism; Quantitative analysis
• ISSN: 0003-2719; 1532-236X
• DOI: 10.1080/00032719808001850

In a weak acidic medium (pH 4.0). a photochemical reaction of cephradine occurs under the irradiation of ultraviolet light, forming a fluorescent product (γ ex = 334 nm, γ em = 442 nm). Based on this fact, a new photochemical fluorimetric method was developed for the determination of cephradine. The linear relationship between the fluorescence intensity and the concentration of cephradine is over the range of 0.01 - 4.0 μg/ml. The detection limit is 0.66 ng/ml (n = 11) and the relative standard deviation is 0.72% (n = 8). This method is simple and sensitive, and has been successfully applied to the determination of cephradine in urine samples. Mechanism of the photochemical reaction was also discussed.