Photochemical Fluorimetric Determination of Cephradine

In a weak acidic medium (pH 4.0). a photochemical reaction of cephradine occurs under the irradiation of ultraviolet light, forming a fluorescent product (γ ex = 334 nm, γ em = 442 nm). Based on this fact, a new photochemical fluorimetric method was developed for the determination of cephradine. The...

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Bibliographic Details
Published inAnalytical letters Vol. 31; no. 3; pp. 439 - 450
Main Authors Cai, Wei-Ping, Ouyang, Yao-Guo, Lin, Xue-Yuan, Xu, Jin-Gou
Format Journal Article
LanguageEnglish
Published Philadelphia, PA Taylor & Francis Group 01.02.1998
Taylor & Francis
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ISSN0003-2719
1532-236X
DOI10.1080/00032719808001850

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Summary:In a weak acidic medium (pH 4.0). a photochemical reaction of cephradine occurs under the irradiation of ultraviolet light, forming a fluorescent product (γ ex = 334 nm, γ em = 442 nm). Based on this fact, a new photochemical fluorimetric method was developed for the determination of cephradine. The linear relationship between the fluorescence intensity and the concentration of cephradine is over the range of 0.01 - 4.0 μg/ml. The detection limit is 0.66 ng/ml (n = 11) and the relative standard deviation is 0.72% (n = 8). This method is simple and sensitive, and has been successfully applied to the determination of cephradine in urine samples. Mechanism of the photochemical reaction was also discussed.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032719808001850