CRISPR/Cas‐Based MicroRNA Biosensors

• Published in: Chemistry : a European journal Vol. 29; no. 16; pp. e202203412 - n/a
• Main Authors: Zhang, Qian, Zhang, Xinyi, Zou, Xiaoran, Ma, Fei, Zhang, Chun‐Yang
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 16.03.2023
• Subjects: biomarker; Biomarkers; biosensor; Biosensors; Chemistry; CRISPR; CRISPR-Cas Systems; CRISPR/Cas; disease diagnosis; Localization; MicroRNAs; miRNA; disease diagnosis; miRNA; biosensor; biomarker; CRISPR/Cas
• ISSN: 0947-6539; 1521-3765; 1521-3765
• DOI: 10.1002/chem.202203412

As important post‐transcriptional regulators, microRNAs (miRNAs) play irreplaceable roles in diverse cellular functions. Dysregulated miRNA expression is implicated in various diseases including cancers, and thus miRNAs have become the valuable biomarkers for disease monitoring. Recently, clustered regularly interspaced short palindromic repeats/CRISPR‐associated (CRISPR/Cas) system has shown great promise for the development of next‐generation biosensors because of its precise localization capability, good fidelity, and high cleavage activity. Herein, we review recent advance in development of CRISPR/Cas‐based biosensors for miRNA detection. We summarize the principles, features, and performance of these miRNA biosensors, and further highlight the remaining challenges and future directions. The construction of efficient biosensors for miRNA assay is essential for disease diagnosis, therapy, and prognosis. CRISPR/Cas system possesses high fidelity, precise localization capability, and efficient cleavage activity, making it attractive in biosensing applications. In this review, the recent advances in the development of CRISPR/Cas‐based biosensors for miRNAs assay are discussed.