Study on gene sensor based on primer extension

Based on the fact that the resonant frequency of a piezoelectric crystal is the function of its surface deposit, and that the primer extends after it hybridizes with the template, the primer extension gene sensor technique was developed. The prominent feature of the technique is that fast and sensit...

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Bibliographic Details
Published inScience China. Life sciences Vol. 40; no. 5; pp. 463 - 469
Main Author 陈誉华 宋今丹 李大为
Format Journal Article
LanguageEnglish
Published China Springer Nature B.V 01.10.1997
Subjects
acid
biosensor
crystal
extension
Genes
hybridization
Monitoring systems
nucleic
piezoelectric
primer
reaction
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ISSN1674-7305
1006-9305
1869-1889
1862-2798
DOI10.1007/BF03183583

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Summary:Based on the fact that the resonant frequency of a piezoelectric crystal is the function of its surface deposit, and that the primer extends after it hybridizes with the template, the primer extension gene sensor technique was developed. The prominent feature of the technique is that fast and sensitive frequency signals are used as the monitoring system of gene hybridization and primer strand extension. Results show that this technique may be used in homologous analysis of nucleic acid, trace DNA detection, and determining the integration of DNA. It may also be used for isolation of target gene, gene mutation analysis, and predicting the location of a gene in its genome.
Bibliography:CHEN Yuhua , SONG Jindan(Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang 110001, China)LI Dawei(Dongyu Electronic Institute of Shenyang, Shenyang 110003, China)
11-5841/Q
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ISSN:1674-7305
1006-9305
1869-1889
1862-2798
DOI:10.1007/BF03183583