Diagnostic Power of Selected Cytokines, MMPs and TIMPs in Ovarian Cancer Patients – ROC Analysis

• Published in: Anticancer research Vol. 39; no. 5; pp. 2575 - 2582
• Main Authors: BĘDKOWSKA, GRAŻYNA EWA, PISKÓR, BARBARA, GACUTA, EWA, ZAJKOWSKA, MONIKA, OSADA, JOANNA, SZMITKOWSKI, MACIEJ, DĄBROWSKA, MILENA, ŁAWICKI, SŁAWOMIR
• Format: Journal Article
• Language: English
• Published: Greece International Institute of Anticancer Research 01.05.2019
• Subjects: Adult; Aged; Biomarkers; Biomarkers, Tumor - blood; CA-125 Antigen - blood; Cancer; Carcinoma, Ovarian Epithelial - blood; Carcinoma, Ovarian Epithelial - pathology; Chemiluminescence; Cytokines; Cytokines - blood; Enzyme-linked immunosorbent assay; Female; Gelatinase B; Gynecology; Healthy Volunteers; Humans; Immunoassay; Kidney diseases; Macrophage colony-stimulating factor; Matrilysin; Membrane Proteins - blood; Metalloproteases - blood; Microparticles; Middle Aged; Neoplasms - blood; Neoplasms - pathology; Ovarian cancer; Plasma levels; Post-menopause; Predictive Value of Tests; Prognosis; Proteins - metabolism; ROC Curve; Statistical analysis; Tissue Inhibitor of Metalloproteinases - blood; Tumor markers; Tumors; Ultrasonic imaging; Vascular endothelial growth factor; TIMPs; epithelial ovarian cancer; Cytokines; tumor markers; MMPs
• ISSN: 0250-7005; 1791-7530; 1791-7530
• DOI: 10.21873/anticanres.13380

The aim of the study was to identify new non-invasive ovarian cancer (OC) tumor markers. In postmenopausal ovarian cancer patients and in a control group (benign ovarian lesions and healthy subjects), preoperative plasma levels of cytokines, metalloproteinases and their tissue inhibitors were determined using ELISA while those of CA125 and HE4 by chemiluminescent microparticle immunoassay methods. The diagnostic sensitivity (SE) value was the highest for HE4 and MMP-7 (78.0%). The diagnostic specificity (SP) for M-CSF, VEGF and MMP-9 was 95.2%, 95.2% and 95.7%, respectively. The highest positive predictive value (PPV) for M-CSF and MMP-9 was ~84.6% and negative predictive value (NPV) for MMP-7 and HE4 was ~87.6%. The biggest areas under the ROC curve were obtained for the combination of VEGF, MMP-7 or MMP-9 with HE4+CA125 (0.9130-0.9234), but not for CA125+HE4 (0.8260). Our research confirms the validity of combining classic markers with new markers to improve the diagnostic power of CA125 and HE4.