Structural Analysis and Activity Evaluation of High Fischer Ratio Oligopeptides from Minced Meat of Skipjack (Katsuwonus pelamis)

• Published in: Journal of aquatic food product technology Vol. 28; no. 10; pp. 1063 - 1075
• Main Authors: Wang, Zhi-Gao, Ying, Xiao-Guo, Wang, Yi-Fan, Yu, Xin-Wei, Luo, Hong-Yu
• Format: Journal Article
• Language: English
• Published: Philadelphia Taylor & Francis 26.11.2019; Taylor & Francis Ltd
• Subjects: Activated carbon; anti-drunken; anti-fatigue; blood; Glycogen; Glycogens; high Fischer ratio oligopeptide; High-performance liquid chromatography; HPLC; hydrolysates; hydrolysis; Katsuwonus pelamis; Liquid chromatography; Meat; Mercury; Minced meat; Molecular weight; Oligopeptides; Pepsin; peptide sequence; Peptides; Righting; Righting reflex; Skipjack (Katsuwonus pelamis); sober-up; Structural analysis; technology; Urea; urea nitrogen
• ISSN: 1049-8850; 1547-0636; 1547-0636
• DOI: 10.1080/10498850.2019.1683927

High Fischer ratio oligo-peptides hydrolysate (DAHFP) from minced meat of Skipjack (Katsuwonus pelamis) were prepared by hydrolysis of pepsin and flavourzyme, then adsorpte on activated carbon. DAHFP was separated by Sephadex G-25 to obtain two fractions with molecular weight of 600 and 1300 kDa, which had the Fischer ratios of 37.52 and 32.08, respectively. The activity of the active component of DAHFP (AC- DAHFP) from combining two fractions was evaluated. Results showed that the maximum extension rate of the loss time of righting reflex was 33.68% and the maximum shortening rate of sober-up time was 29.98%. The difference in content of hepatic glycogen (HG) and blood urea nitrogen (BUN) was significant (P < .05) among the high, moderate dose groups and the blank group. All results indicated that AC- DAHFP could be used in anti-drunken, sobering-up and anti-fatigue. Additionally, peptides were isolated from two fractions using C18 reversed phase high-performance liquid chromatography (RP-HPLC). Five of the peptide sequences were detected as Leu-Leu-Tyr-Ala-Ala-Gly-Ala; Leu-Leu-Asp-Val-Tyr; Leu-Leu-Phe-Thr-Thr-Gln; Leu-Leu-Tyr-Val-Tyr-Gln; Tyr-Val-Ser-Trp-Lys-Leu.