Identification and characterization of EST-SSRs and cpSSRs in endangered Cycas hainanensis

• Published in: Conservation genetics Vol. 9; no. 4; pp. 1079 - 1081
• Main Authors: Wang, Zheng-Feng, Ye, Wan-Hui, Cao, Hong-Lin, Li, Zhong-Chao, Peng, Shao-Lin
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.08.2008; Springer Netherlands; Springer Nature B.V
• Subjects: alleles; Animal Genetics and Genomics; Biodiversity; Biomedical and Life Sciences; Chloroplasts; Conservation biology; Conservation Biology/Ecology; Cycas; Ecology; Endangered & extinct species; Evolutionary Biology; Flowers & plants; Genetic diversity; genetic variation; Life Sciences; microsatellite repeats; Plant Genetics and Genomics; Population genetics; Population structure; Technical Note; Primer; Expressed sequence tag; Simple sequence repeat; Chloroplast
• ISSN: 1566-0621; 1572-9737
• DOI: 10.1007/s10592-007-9461-5

We report on the identification and characterization of six EST-linked simple sequence repeats (EST-SSRs) and chloroplast SSRs (cpSSRs) in endangered Cycas hainanensis. The number of alleles ranged from two to eight for EST-SSRs, two to three for cpSSRs. Observed and expected heterozygosities ranged from 0.042 to 0.417 and 0.042 to 0.811 for EST-SSRs, respectively. Expected heterozygosities ranged from 0.156 to 0.457 for cpSSRs. All these markers gave successful cross-species amplification in C. fairylakea. These markers will allow analyses of the baseline genetic variability and population structure of C. hainanensis to provide strategies for effective conservation and management.