Inhibitory Effects of miR-146b-5p on Cell Migration and Invasion of Pancreatic Cancer by Targeting MMP16

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 31; no. 4; pp. 509 - 514
• Main Author: 林凡 王欣 揭志刚 洪晓泉 李旭 王敏 俞亚红
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.08.2011
• Subjects: Cell Line, Tumor; Cell Movement - genetics; Humans; Matrix Metalloproteinase 16 - genetics; Matrix Metalloproteinase 16 - metabolism; Medicine; Medicine & Public Health; MicroRNAs - genetics; miRNA; Neoplasm Invasiveness - genetics; Pancreatic Neoplasms - genetics; Pancreatic Neoplasms - metabolism; 侵袭; 基质金属蛋白酶; 定位; 抑制作用; 细胞迁移; 胰腺癌; 过度表达; migration; miR-146b-5p; matrix metalloproteinase; pancreatic cancer; invasion
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-011-0481-5

Previous studies have shown that miRNAs participate in a wide range of biological functions and play important roles in various human diseases including cancer.We found miR-146b-5p significantly dysregulated in human pancreatic cancer cells by qRT-PCR.To demonstrate its function and regulation mechanism,we overexpressed miR-146-5p by transfecting the mimics.Our data showed that miR-146b-5p overexpression significantly reduced the abilities of migration and invasion of MIA PaCa-2 pancreatic cancer cells.Furthermore,we found that matrix metalloproteinase 16（MMP16） was a downstream target of miR-146b-5p by dual-luciferase reporter assay.Altogether,our findings suggest that miR-146b-5p may be involved in pancreatic cancer cell migration and invasion by targeting MMP16,and miR-146b-5p may be a potential therapeutic target for the pancreatic cancer.