Comparative Study on the Effects of Self-made Liver Preservation Solution on Secretion of ICAM-1 and NO in Rats

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 28; no. 1; pp. 77 - 79
• Main Author: 王世明 戴鹏 徐钧 杨镇
• Format: Journal Article
• Language: English
• Published: Wuhan Huazhong University of Science and Technology 01.02.2008
• Subjects: Adenosine - chemistry; Adenosine - pharmacology; Allopurinol - chemistry; Allopurinol - pharmacology; Animals; Glutathione - chemistry; Glutathione - pharmacology; Insulin - chemistry; Insulin - pharmacology; Intercellular Adhesion Molecule-1 - biosynthesis; Liver - metabolism; Liver - pathology; Liver Transplantation - instrumentation; Liver Transplantation - methods; Male; Medicine; Medicine & Public Health; Nitric Oxide - biosynthesis; Organ Preservation - instrumentation; Organ Preservation - methods; Organ Preservation Solutions - chemistry; Organ Preservation Solutions - pharmacology; Raffinose - chemistry; Raffinose - pharmacology; Rats; Rats, Wistar; Reperfusion; Time Factors; 保存方法; 细胞黏附因子; 肝移植; nitric oxide; liver transplantation; preservation solution; intercellular adhesion molecule-1
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-008-0119-4

In order to study the effect of self-made liver preservation solution on liver preservation by comparing with UW solution and HC-A solution, the self-made liver preservation solution （SM） and perfusion solution were prepared under the aseptic conditions. The isolated non-circulated perfusion rat liver model was established. According to the different preservation solutions, the rats were randomly divided into UW group, SM group and HC-A group. The three groups were divided into 6 subgroups according to the preservation duration （n=6 in each group）. The transferase in liver perfusion solution and intercellular adhesion molecule-1 （ICAM-1） and nitric oxide （NO） in liver tissues were determined at 2, 8 and 24 h respectively. The results showed that the levels of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） had no significant difference between SM group and UW group, but significantly lower than in HC-A group. The levels of ICAM-1 and NO were increased simultaneously in SM group and UW group （P〉0.05）, but there was significant difference as compared with HC-A group （P〈0.05）. At the same time point, the level of ICAM-1 was higher in SM group than in UW group, but NO was lower. The preservation effect of SM solution is the same as UW solution, but better than HC-A solution.