Detection of galectin-3 in patients with inflammatory bowel diseases: new serum marker of active forms of IBD?

• Published in: Inflammation research Vol. 58; no. 8; pp. 503 - 512
• Main Authors: Frol’ová, Lenka, Smetana, Karel, Borovská, Dana, Kitanovičová, Andrea, Klimešová, Klára, Janatková, Ivana, Malíčková, Karin, Lukáš, Milan, Drastich, Pavel, Beneš, Zdeněk, Tučková, Ludmila, Manning, Joachim C., André, Sabine, Gabius, Hans-Joachim, Tlaskalová-Hogenová, Helena
• Format: Journal Article
• Language: English
• Published: Basel SP Birkhäuser Verlag Basel 01.08.2009; Springer Nature B.V
• Subjects: Allergology; Animals; Biomarkers; Biomedical and Life Sciences; Biomedicine; Blotting, Western; Colitis - chemically induced; Colitis, Ulcerative - blood; Colon - metabolism; Crohn Disease - blood; Dermatology; Dextran Sulfate; Electrophoresis, Polyacrylamide Gel; Escherichia coli - metabolism; Female; Fluorescein-5-isothiocyanate; Fluorescent Dyes; Galectin 3 - blood; Humans; Immunohistochemistry; Immunology; Inflammatory Bowel Diseases - blood; Inflammatory Bowel Diseases - chemically induced; Inflammatory Bowel Diseases - diagnosis; Lectins - metabolism; Lipopolysaccharide Receptors - analysis; Lipopolysaccharide Receptors - metabolism; Mice; Mice, Inbred BALB C; Neurology; Original Research Paper; Pharmacology/Toxicology; Rheumatology; Mucosal immunity; Galectin-3; Disease marker; Crohn’s disease; Ulcerative colitis
• ISSN: 1023-3830; 1420-908X; 1420-908X
• DOI: 10.1007/s00011-009-0016-8

Objective It is an open question whether multifunctional galectin-3 can be a serum marker in inflammatory bowel disease. Methods Western blots and commercial ELISA detected and quantitated the lectin immunocytochemistry using double labeling localized it in tissue sections. Results Serum concentrations were significantly increased in specimen of patients with active and remission-stage ulcerative colitis and Crohn’s disease, associated with emerging positivity of CD14 + cells. Conclusion Enhanced concentration of galectin-3 in serum reflects presence of disease and points to its involvement in the pathogenesis.