Ytterbium ion promotes apoptosis of primary mouse bone marrow stromal cells

One of the main target organs for the lanthanides （Ln） is bone. Previous studies revealed that ytterbium （Yb） produced damage to the skeletal system in vivo. But the effects of Yb3＋ on bone marrow stromal cells （BMSCs） in vitro had not been reported. In this paper, cell viability, apoptosis, mitocho...

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Bibliographic Details
Published inJournal of rare earths Vol. 33; no. 4; pp. 445 - 452
Main Author 戴春燕 陈士柱 王朝 张良 葛昆 张金超
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.04.2015
Subjects
Apoptosis
Bone marrow
bone marrow stromal cells
Bones
In vitro testing
precipitation
Rare earth metals
rare earths
Surgical implants
Viability
Ytterbium
乳酸脱氢酶
小鼠
线粒体膜电位
细胞凋亡
细胞活力
镱离子
骨髓基质干细胞
骨髓基质细胞
bone marrow stromal cells
precipitation
apoptosis
ytterbium
rare earths
Online AccessGet full text
ISSN1002-0721
2509-4963
DOI10.1016/S1002-0721(14)60439-7

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Summary:One of the main target organs for the lanthanides （Ln） is bone. Previous studies revealed that ytterbium （Yb） produced damage to the skeletal system in vivo. But the effects of Yb3＋ on bone marrow stromal cells （BMSCs） in vitro had not been reported. In this paper, cell viability, apoptosis, mitochondrial membrane potential （MMP）, reactive oxygen species （ROS） and lactate dehy- drogenase （LDH） were measured in order to study the effects of Yb3＋ on BMSCs. The results indicated that Yb3＋ displayed a slight positive effect on the BMSCs viability at concentrations of 1 x 104, 1 × 105, and 1 x 104 mol/L, but turned to decrease the viability of BMSCs at the highest concentration of 1 × 103 mol/L for 24, 48 and 72 h. Yb3＋ at 1 x 103 mol/L promoted apoptosis of BMSCs, in- creased the levels of ROS and LDH, and decreased MMP in BMSCs. It suggested that the precipitate of YbPO4 might decrease the viability of BMSCs. Yb3＋ induced the apoptosis of BMSCs via mitochondrial pathway. The results might be useful for more rational application of Yb-based compounds in the future.
Bibliography:DAI Chunyan , CHEN Shizhu , WANG Chao , ZHANG Liang , GE Kun ZHANG Jinchao . Chemical Biology Key Laboratory of Hebei Province, College of Chemistry ＆ Environmental Science, Hebei University, Baoding 071002, China 2. Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University, Baoding 071002, China 3. Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry ＆ Chemical Engineering, Hainan Normal University, Haikou 571158, China
ytterbium; bone marrow stromal cells; precipitation; apoptosis; rare earths
11-2788/TF
One of the main target organs for the lanthanides （Ln） is bone. Previous studies revealed that ytterbium （Yb） produced damage to the skeletal system in vivo. But the effects of Yb3＋ on bone marrow stromal cells （BMSCs） in vitro had not been reported. In this paper, cell viability, apoptosis, mitochondrial membrane potential （MMP）, reactive oxygen species （ROS） and lactate dehy- drogenase （LDH） were measured in order to study the effects of Yb3＋ on BMSCs. The results indicated that Yb3＋ displayed a slight positive effect on the BMSCs viability at concentrations of 1 x 104, 1 × 105, and 1 x 104 mol/L, but turned to decrease the viability of BMSCs at the highest concentration of 1 × 103 mol/L for 24, 48 and 72 h. Yb3＋ at 1 x 103 mol/L promoted apoptosis of BMSCs, in- creased the levels of ROS and LDH, and decreased MMP in BMSCs. It suggested that the precipitate of YbPO4 might decrease the viability of BMSCs. Yb3＋ induced the apoptosis of BMSCs via mitochondrial pathway. The results might be useful for more rational application of Yb-based compounds in the future.
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ISSN:1002-0721
2509-4963
DOI:10.1016/S1002-0721(14)60439-7