Extracellular matrix of lung scaffolds submitted to different means of sterilization: a systematic review [version 1; peer review: 1 approved, 2 approved with reservations, 1 not approved]

• Published in: F1000 research Vol. 13; p. 554
• Main Authors: Moura, Ricardo S., Afonso, Joao Pedro R., Fonseca, Adriano L., Cereta, Andressa D., Mello, Diego A. C. P. G., Oliveira, Miria C., Oliveira-Silva, Iransé, Oliveira, Rodrigo F., Oliveira, Deise A. A. P., Vieira, Rodolfo P., Palma, Renata K., Insalaco, Giuseppe, Oliveira, Luis Vicente Franco
• Format: Journal Article
• Language: English
• Published: England Faculty of 1000 Ltd 2024; F1000 Research Ltd
• Subjects: Animals; Bias; Chronic obstructive pulmonary disease; Decellularization; eng; Engineering; Extracellular Matrix; Humans; Intervention; Keywords; Laboratory animals; Lasers; Light emitting diodes; Lung; Lung diseases; Lungs; Medical Subject Headings-MeSH; Photodynamic therapy; Recellularization; Respiratory diseases; Scaffolds; Stem cells; Sterilization; Sterilization - methods; Systematic review; Tissue Engineering - methods; Tissue Scaffolds; Extracellular matrix; Scaffolds; Recellularization; Stem cells; Decellularization; Sterilization
• ISSN: 2046-1402; 2046-1402
• DOI: 10.12688/f1000research.147670.1

Chronic respiratory diseases often necessitate lung transplantation due to irreversible damage. Organ engineering offers hope through stem cell-based organ generation. However, the crucial sterilization step in scaffold preparation poses challenges. This study conducted a systematic review of studies that analysed the extracellular matrix (ECM) conditions of decellularised lungs subjected to different sterilisation processes. A search was performed for articles published in the PubMed, Web of Sciences, Scopus, and SciELO databases according to the PRISMA guidelines. Overall, five articles that presented positive results regarding the effectiveness of the sterilisation process were selected, some of which identified functional damage in the ECM. Was possible concluded that regardless of the type of agent used, physical or chemical, all of them demonstrated that sterilisation somehow harms the ECM. An ideal protocol has not been found to be fully effective in the sterilisation of pulmonary scaffolds for use in tissue and/or organ engineering.