Astrocytic EphB3 receptors regulate d-serine-gated synaptic plasticity and memory

• Published in: Progress in neurobiology Vol. 248; p. 102747
• Main Authors: Langlais, Valentin Clément, Mountadem, Sarah, Benazzouz, Ines, Amadio, Aurélie, Matos, Marco, Jourdes, Aurélie, Cannich, Astrid, Julio-Kalajzic, Francisca, Belluomo, Ilaria, Matias, Isabelle, Maitre, Marlène, Lesté-Lasserre, Thierry, Marais, Sébastien, Avignone, Elena, Marsicano, Giovanni, Bellocchio, Luigi, Oliet, Stéphane Henri Richard, Panatier, Aude
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2025
• Subjects: Animals; Astrocyte; Astrocytes - drug effects; Astrocytes - metabolism; D-serine; Ephrin; Hippocampus - cytology; Hippocampus - metabolism; Hippocampus - physiology; Long-Term Potentiation - drug effects; Long-Term Potentiation - physiology; LTP; Male; Memory; Memory - drug effects; Memory - physiology; Mice; Mice, Inbred C57BL; Neuronal Plasticity - drug effects; Neuronal Plasticity - physiology; NMDA receptor; Receptor, EphB3 - metabolism; Receptors, N-Methyl-D-Aspartate - metabolism; Serine - metabolism; Serine - pharmacology; Synapses - drug effects; Synapses - metabolism; Synapses - physiology; LTP; D-serine; Ephrin; Astrocyte; NMDA receptor; Memory
• ISSN: 0301-0082; 1873-5118; 1873-5118
• DOI: 10.1016/j.pneurobio.2025.102747

The activation of classical NMDA receptors (NMDARs) requires the binding of a co-agonist in addition to glutamate. Whereas astrocytic-derived d-serine was shown to play such a role at CA3-CA1 hippocampal synapses, the exact mechanism by which neurons interact with neighboring astrocytes to regulate synaptic d-serine availability remains to be fully elucidated. Considering the close anatomical apposition of astrocytic and neuronal elements at synapses, the aforementioned process is likely to involve cells adhesion molecules. One very likely candidate could be the astrocytic EphB3 receptor and its neuronal partner, ephrinB3. Here, we first showed in acute hippocampal slices from adult mice that stimulation of EphB3 receptors with exogenous ephrinB3 increased d-serine availability at CA3-CA1 synapses, resulting in an increased NMDAR activity. Conversely, inhibiting endogenous EphB3 receptors caused an impairment of both synaptic NMDAR activity and NMDAR-dependent long-term synaptic potentiation (LTP), effects that could be rescued by exogenous d-serine. Most interestingly, knocking down EphB3 receptors specifically in astrocytes yielded a similar impairment in hippocampal plasticity and, most importantly, caused a deficit in novel object recognition memory. Altogether, our data thus indicate that EphB3 receptors in hippocampal astrocytes play a key role in regulating synaptic NMDAR function, activity-dependent plasticity and memory. •Astrocytic EphB3 receptors regulate synaptic d-serine availability.•NMDA receptors activity and LTP induction at CA3-CA1 synapses depend on astrocytic EphB3 receptors.•EphB3 receptors located on astrocytes are important for novel object recognition memory.