Complete sequence and organization of the Serratia marcescens biotin operon

• Published in: Microbiology (Society for General Microbiology) Vol. 142; no. 11; pp. 3295 - 3303
• Main Authors: Sakurai, Naoki, Akatsuka, Hiroyuki, Kawai, Eri, Imai, Yuji, Komatsubara, Saburo
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.11.1996; Society for General Microbiology
• Subjects: Amino Acid Sequence; Bacterial Proteins - genetics; Bacteriology; Base Sequence; Biological and medical sciences; Biotin - biosynthesis; Cloning, Molecular; DNA Primers - genetics; DNA, Bacterial - genetics; Escherichia coli - genetics; Escherichia coli Proteins; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genetics; Lac Operon; Microbiology; Molecular Sequence Data; Open Reading Frames; Operon; Promoter Regions, Genetic; Sequence Homology, Amino Acid; Serratia marcescens - enzymology; Serratia marcescens - genetics; Serratia marcescens - metabolism; Sulfurtransferases; Transaminases; Regulation(control); Nucleotide sequence; Operon; DNA; Serratia marcescens; Bacteria; Gene organization; Biosynthesis; Biotin; Gene expression; Aminoacid sequence; Enterobacteriaceae
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/13500872-142-11-3295

Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co. Ltd, 16-89 Kashima-3-chome, Yodogawa-ku, Osaka 532, Japan ABSTRACT The nucleotide sequence of the biotin ( bio ) operon of wild-type Serratia marcescens Sr41 was determined. Five ORFs were identified to encode BioA (7,8-diaminopelargonic acid aminotransferase), BioB (biotin synthase), BioF (7-keto-8-aminopelargonic acid synthase), BioC (an enzyme catalysing the synthesis of pimeloyl-CoA) and BioD (dethiobiotin synthase), in this order. The operon was deduced to be transcribed divergently to the left into bioA and to the right into the bioBFCD genes. The promoters and a common predicted operator for both bioA and bioBFCD genes were located between the bioA and bioB genes. The predicted amino acid sequences of these enzymes were similar to the sequences of the corresponding enzymes of Escherichia coli. Analysis of expression of the lacZ structural gene fused with the bioA and bioB promoters revealed that the biotin operon was subject to biotin-mediated feedback repression. 1 Author for correspondence: Naoki Sakurai. Tel: +81 48 433 2737. Fax: +81 48 433 2734. e-mail: nsakurai@tanabe.co.jp Keywords: biotin biosynthesis, biotin operon, Serratia marcescens Present address: Lead Generation Research Laboratory at Toda, Tanabe Seiyaku Co. Ltd, 2-50, Kawagishi-2-chome, Toda-shi, Saitama 335, Japan.