New putative cryptic species detection and genetic network analysis of Bemisia tabaci (Hempitera: Aleyrodidae) in China based on mitochondrial COI sequences

• Published in: Mitochondrial DNA. Part A. DNA mapping, sequencing, and analysis Vol. 29; no. 3; pp. 474 - 484
• Main Authors: Hu, Jian, Zhang, Xiaoyun, Jiang, Zhilin, Zhang, Feifei, Liu, Yuanyuan, Li, Zhan, Zhang, Zhongkai
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 03.04.2018
• Subjects: Animals; Bayes Theorem; Bemisia tabaci; Biodiversity; China; cryptic species; Electron Transport Complex IV - genetics; haplotype network; Hemiptera - classification; Hemiptera - genetics; Insect Proteins - genetics; Mediterranean Sea; mitochondrial COI; phylogenetic tree; Phylogeny; Phylogeography; Sequence Analysis, DNA - methods; phylogenetic tree; haplotype network; mitochondrial COI; Bemisia tabaci; cryptic species
• ISSN: 2470-1394; 2470-1408; 2470-1408
• DOI: 10.1080/24701394.2017.1307974

The whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a cryptic species complex and widely distributed throughout tropical and subtropical regions. To understand the B. tabaci cryptic species diversity in China more comprehensively, in the year 2014 and 2016, a large-scale sampling was conducted from the famous biodiversity hotspot of China, Yunnan province. Mitochondrial cytochrome oxidase I gene sequences were used to identify new putative cryptic species. Phylogenetic analyses were performed using Bayesian methods to evaluate the position of new cryptic species in the context of the B. tabaci diversity in Asia. Two new cryptic species, China 5 and Asia V were identified. In total, 19 B. tabaci cryptic species are present in China, two invasive (MED and MEAM1) and 17 indigenous. A new sibling species of B. tabaci was first defined and reported. Based on the mtCOI sequences and haplotype network analyses, the genetic diversity of MED was far higher than MEAM1. We confirmed the exotic MED was originated from the western Mediterranean regions and first invaded into Yunnan, China. The genetic structures of other four indigenous species (Asia I, Asia II 1, Asia II 6, and China 1) with relatively wide distribution ranges in China were also discussed.