Assessment of genetic factors for type 2 diabetes mellitus

• Published in: International journal of molecular medicine Vol. 18; no. 2; pp. 299 - 308
• Main Authors: Yamada, Yoshiji, Matsuo, Hitoshi, Segawa, Tomonori, Watanabe, Sachiro, Kato, Kimihiko, Kameyama, Takashi, Yokoi, Kiyoshi, Ichihara, Sahoko, Metoki, Norifumi, Yoshida, Hidemi, Satoh, Kei, Nozawa, Yoshinori
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.08.2006
• Subjects: Aged; Blood Glucose - metabolism; Diabetes Mellitus, Type 2 - genetics; Female; Genetic Predisposition to Disease; Genotype; Humans; Japan; Male; Middle Aged; Multivariate Analysis; Polymorphism, Genetic; Regression Analysis; Risk Factors; Japan
• ISSN: 1107-3756; 1791-244X
• DOI: 10.3892/ijmm.18.2.299

The purpose of the present study was to identify gene polymorphisms for reliable assessment of genetic factors for type 2 diabetes mellitus. The study population comprised 4853 unrelated Japanese individuals (2688 men, 2165 women), including 1489 subjects with type 2 diabetes mellitus (969 men, 520 women) and 3364 controls (1719 men, 1645 women). The genotypes for 148 polymorphisms of 124 candidate genes were determined with a method that combines polymerase chain reaction and sequence-specific oligonucleotide probes with suspension array technology. Sixteen polymorphisms were related (p<0.05) to the prevalence of type 2 diabetes mellitus as determined by the chi-square test. Multivariable logistic regression analysis with adjustment for age, sex, and the prevalence of smoking revealed that, among these polymorphisms, the -603A↷G polymorphism of the gene for coagulation factor III (F3) was significantly (p<0.001) associated with the prevalence of type 2 diabetes mellitus, with the -603G allele representing a risk factor for this condition. A stepwise forward selection procedure demonstrated that F3 genotype (GG versus AA + AG) significantly (p<0.001) and independently affected the prevalence of type 2 diabetes mellitus. Genotype for F3 may prove reliable for assessment of genetic factors for type 2 diabetes mellitus. Determination of the genotype for this gene may contribute to personalized prevention of this condition.