Human Umbilical Cord Mesenchymal Stem Cells in Combination with Hyaluronic Acid Ameliorate the Progression of Knee Osteoarthritis

The aim of this study is to evaluate the feasibility and usefulness of the human umbilical cord mesenchymal stem cells (hUC-MSCs) and hyaluronan acid (HA) combination to attenuate osteoarthritis progression in the knee while simultaneously providing some insights on the mitigation mechanism. In vitr...

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Published inApplied sciences Vol. 11; no. 14; p. 6650
Main Authors Wu, Jia-Lin, Wong, Pei-Chun, Ho, Chung-Wei, Chen, Chien-Han, Liao, Kuan-Ya, Lovel, Ronald, Wu, Tang Bo-Chung, Chang, Wen-Ying, Lee, Yan-Zhang, Lin, Willie
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.07.2021
Subjects
antioxidation
Arthritis
Carbon dioxide
Cartilage
Cell culture
Cytokines
DKK-1
Fibroblasts
Growth factors
Humidity
hyaluronan acid
Hyaluronic acid
Inflammation
Knee
mesenchymal stem cell
Molecular weight
Osteoarthritis
Stem cells
Umbilical cord
United States > US
Taiwan
Online AccessGet full text
ISSN2076-3417
2076-3417
DOI10.3390/app11146650

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Summary:The aim of this study is to evaluate the feasibility and usefulness of the human umbilical cord mesenchymal stem cells (hUC-MSCs) and hyaluronan acid (HA) combination to attenuate osteoarthritis progression in the knee while simultaneously providing some insights on the mitigation mechanism. In vitro, the effect of hUC-MSCs with HA treatment on chondrocyte cell viability and the cytokine profile were analyzed. Additionally, the antioxidation capability of hUC-MSCs-CM (conditioned medium) with HA towards H2O2-induced chondrocyte cell damage was evaluated. The HA addition increased the hUC-MSC antioxidation capability and cytokine secretion, such as Dickkopf-related protein 1 (DKK-1) and hepatocyte growth factor (HGF), while no adverse effect on the cell viability was observed. In vivo, the intra-articular injection of hUC-MSCs with HA to a mono-iodoacetate (MIA)-induced knee osteoarthritis (KOA) rat model was performed and investigated. Attenuation of the KOA progression in the MIA-damaged rat model was seen best in hUC-MSCs with a HA combination compared to the vehicle control or each individual element. Combining hUC-MSCs and HA resulted in a synergistic effect, such as increasing the cell therapeutic capability while incurring no observable adverse effects. Therefore, this combinatorial therapy is feasible and has promising potential to ameliorate KOA progression.
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ISSN:2076-3417
2076-3417
DOI:10.3390/app11146650