Preferential extrachromosomal localization of exogenous DNA in transgenic silkworm Bombyx mori L

• Published in: Molecular & general genetics Vol. 236; no. 2-3; p. 326
• Main Authors: Nikolaev, A.I. (Russian Academy of Sciences, Moscow (Russian Federation). V.A. Engelhardt Inst. of Molecular Biology), Tchkonia, T.T, Kafiani-Eristavi, C.A, Tarantul, V.Z
• Format: Journal Article
• Language: English
• Published: Germany 1993
• Subjects: ADN; ANIMAL TRANSGENIQUE; ANIMALES TRANSGENICOS; Animals; Animals, Genetically Modified; Avian Sarcoma Viruses - genetics; Bombyx - embryology; Bombyx - genetics; BOMBYX MORI; Crosses, Genetic; DNA rearrangement; Embryo, Nonmammalian - physiology; Extrachromosomal Inheritance; Gene transfer; Genes, Viral - genetics; HEREDITE; HERENCIA GENETICA; Microinjections; Plasmids - genetics; Repetitive Sequences, Nucleic Acid - genetics; Restriction Mapping; TRANSFERENCIA DE GENES; TRANSFERT DE GENE; Transgenic silkworm; Virus Integration - genetics
• ISSN: 0026-8925
• DOI: 10.1007/BF00277129

Transgenic silkworms (Bombyx mori L.) were obtained by microinjection of plasmid pPrC-LTR 1.5, which carris 1.5 DNA copies of Rous sarcoma virus (RSV) long terminal repeats (LTRs) inserted in the vector pBR322. The transgene was transmitted over the three generations obtained up to now. Most of the exogenous DNA failed to integrate into the genome and persisted as an extrachromosomal element that is subject to rearrangements. Plasmids carrying only part of the input DNA together with fragments of silkworm DNA were rescued from the transgenic animals. One of the rescued plasmids contained a sequence which belongs to a family of evolutionarily conserved repeated sequences.