New gregarine species (Apicomplexa) from tunicates show an evolutionary history of host switching and suggest a problem with the systematics of Lankesteria and Lecudina

• Published in: Journal of invertebrate pathology Vol. 183; p. 107622
• Main Authors: Iritani, Davis, Banks, Jonathan C., Webb, Stephen C., Fidler, Andrew, Horiguchi, Takeo, Wakeman, Kevin C.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.07.2021
• Subjects: Apicomplexa; Ascidiacea; Eugregarinorida; Evolution; gregarines; invertebrates; Miozoa; Morphology; new species; parasitism; phylogeny; Polychaeta; Systematics; Evolution; Apicomplexa; Systematics; Ascidiacea; Eugregarinorida; Morphology
• ISSN: 0022-2011; 1096-0805; 1096-0805
• DOI: 10.1016/j.jip.2021.107622

[Display omitted] •New gregarine parasites (Apicomplexa) were discovered from sea squirts.•Species were described using morphology and SSU rDNA.•Data revealed a history of host switching.•Two major genera were found to be taxonomically problematic. Apicomplexa (sensu stricto) are a diverse group of obligate parasites to a variety of animal species. Gregarines have been the subject of particular interest due to their diversity, phylogenetically basal position, and more recently, their symbiotic relationships with their hosts. In the present study, four new species of marine eugregarines infecting ascidian hosts (Lankesteria kaiteriteriensis sp. nov., L. dolabra sp. nov., L. savignyii sp. nov., and L. pollywoga sp. nov.) were described using a combination of morphological and molecular data. Phylogenetic analysis using small subunit rDNA sequences suggested that gregarines that parasitize ascidians and polychaetes share a common origin as traditionally hypothesized by predecessors in the discipline. However, Lankesteria and Lecudina species did not form clades as expected, but were instead intermixed amongst each other and their respective type species in the phylogeny. These two major genera are therefore taxonomically problematic. We hypothesize that the continued addition of new species from polychaete and tunicate hosts as well as the construction of multigene phylogenies that include type-material will further dissolve the currently accepted distinction between Lankesteria and Lecudina. The species discovered and described in the current study add new phylogenetic and taxonomic data to the knowledge of marine gregarine parasitism in ascidian hosts.