Neutron scattering method for studying bound water in live tissue application to plant leaves
Illustrative neutron scattering studies are presented of rotational modes of bound H 2O in live leaves of Ficus elastica decora, Philodendron cordatum and Peperomia obtusifolia. Interpretation of data is based on the hypothesis that hindrance of rotational motion should result in shifts to higher fr...
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Published in | Biochimica et biophysica acta Vol. 714; no. 1; pp. 65 - 73 |
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Main Author | |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
12.01.1982
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Subjects | |
Online Access | Get full text |
ISSN | 0304-4165 0006-3002 1872-8006 1878-2434 |
DOI | 10.1016/0304-4165(82)90127-1 |
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Summary: | Illustrative neutron scattering studies are presented of rotational modes of bound H
2O in live leaves of
Ficus elastica decora, Philodendron cordatum and
Peperomia obtusifolia. Interpretation of data is based on the hypothesis that hindrance of rotational motion should result in shifts to higher frequency. Such shifts are verified by comparing scattering from water with that from ice and cytosine monohydrate. Statistical analysis of the scattering from plant leaves is based on the assumption that least-squares fitting can be carried out using variable percentages of ice and water spectra to describe hydrate and free water fractions. It is found that the amount of water present as a 4-fold bonded hydrate is very small, being of the order of a few percent or less. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0304-4165 0006-3002 1872-8006 1878-2434 |
DOI: | 10.1016/0304-4165(82)90127-1 |