Angiogenesis-related cytokines in serum of proliferative diabetic retinopathy patients before and after vitrectomy

• Published in: International journal of ophthalmology Vol. 5; no. 6; pp. 726 - 730
• Main Authors: Li, Shuang, Fu, Xun-An, Zhou, Xiao-Fang, Chen, You-Yan, Chen, Wei-Qun
• Format: Journal Article
• Language: English
• Published: China International Journal of Ophthalmology Press 2012; Press of International Journal of Ophthalmology (IJO PRESS)
• Subjects: angiogenesis; assay; Clinical Research; cytokine; diabetic; enzyme-linked; enzyme-linked immunosorbent assay; immunosorbent; proliferative; proliferative diabetic retinopathy; retinopathy; vitrectomy; cytokine; vitrectomy; proliferative diabetic retinopathy; angiogenesis; enzyme-linked immunosorbent assay
• ISSN: 2222-3959; 2227-4898
• DOI: 10.3980/j.issn.2222-3959.2012.06.14

AIM: To evaluate serum concentrations of angiogenesis- related cytokines in proliferative diabetic retinopathy (PDR) before and after vitrectomy. METHODS: Serum samples were collected from 30 PDR patients with varying severity before and after vitrectomy. Serum concentrations of vascular endothelial growth factor (VEGF), pigment epithelium-derived factor (PEDF), interleukin-8 (IL-8) and interferon-inducible protein-10 (IP-10) were determined by enzyme-linked immunosorbent assays(ELISA). RESULTS: Serum concentrations of VEGF, PEDF, IL-8 and IP-10 were significantly higher in PDR patients than that in controls, respectively (P <0.05). VEGF concentration decreased significantly in postoperative samples than that in preoperative samples (P <0.05). The concentrations of PEDF, IL-8 and IP-10 did not exhibit significant changes after vitrectomy. CONCLUSION: Elevated cytokines levels in serum may be diagnostically useful in PDR. Angiogenesis-related cytokines play important roles in the development of PDR, and would instruct the risk assessment of pathogenetic condition in PDR patients.