Ethanol exposure perturbs sea urchin development and disrupts developmental timing

• Published in: Developmental biology Vol. 493; pp. 89 - 102
• Main Authors: Rodríguez-Sastre, Nahomie, Shapiro, Nicholas, Hawkins, Dakota Y., Lion, Alexandra T., Peyreau, Monique, Correa, Andrea E., Dionne, Kristin, Bradham, Cynthia A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2023
• Subjects: Animals; Ectoderm; Embryo, Nonmammalian - metabolism; Ethanol; Ethanol - toxicity; FAS; Gene Expression Regulation, Developmental; Hedgehog; Mesenchymal Stem Cells - metabolism; Retinoic acid; Sea Urchins; Timing; FAS; Hedgehog; Sea urchins; Timing; Ethanol; Retinoic acid
• ISSN: 0012-1606; 1095-564X; 1095-564X
• DOI: 10.1016/j.ydbio.2022.11.001

Ethanol is a known vertebrate teratogen that causes craniofacial defects as a component of fetal alcohol syndrome (FAS). Our results show that sea urchin embryos treated with ethanol similarly show broad skeletal patterning defects, potentially analogous to the defects associated with FAS. The sea urchin larval skeleton is a simple patterning system that involves only two cell types: the primary mesenchymal cells (PMCs) that secrete the calcium carbonate skeleton and the ectodermal cells that provide migratory, positional, and differentiation cues for the PMCs. Perturbations in RA biosynthesis and Hh signaling pathways are thought to be causal for the FAS phenotype in vertebrates. Surprisingly, our results indicate that these pathways are not functionally relevant for the teratogenic effects of ethanol in developing sea urchins. We found that developmental morphology as well as the expression of some ectodermal and PMC genes was delayed by ethanol exposure. Temporal transcriptome analysis revealed significant impacts of ethanol on signaling and metabolic gene expression, and a disruption in the timing of GRN gene expression that includes both delayed and precocious gene expression throughout the specification network. We conclude that the skeletal patterning perturbations in ethanol-treated embryos likely arise from a loss of temporal synchrony within and between the instructive and responsive tissues. [Display omitted] •Ethanol perturbs sea urchin skeletal patterning.•Ethanol-mediated phenotypes are not mediated by retinoic acid.•Ethanol desynchronizes specification network gene expression timing.