Identification of novel candidate genes for globin regulation in erythroid cells containing large deletions of the human β-globin gene cluster

• Published in: Blood cells, molecules, & diseases Vol. 37; no. 2; pp. 82 - 90
• Main Authors: de Andrade, Tiago Gomes, Peterson, Kenneth R., Cunha, Anderson F., Moreira, Luciana Sarmento, Fattori, André, Saad, Sara T.O., Costa, Fernando Ferreira
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2006
• Subjects: Chromatin remodeling; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Erythroid Cells - metabolism; Female; Fetal Hemoglobin - genetics; Gene Expression Profiling; Gene Library; Globin; Globins - genetics; Globins - metabolism; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; HPFH; Humans; Kruppel-Like Transcription Factors; Male; Multigene Family; Nuclear Proteins; Nucleic Acid Hybridization - methods; Proteins - genetics; Proteins - metabolism; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Messenger - genetics; Sensitivity and Specificity; Sequence Deletion; Thalassemia; Thalassemia - genetics; Transcription; Transcription Factors - genetics; Transcription Factors - metabolism; Transcription, Genetic; Globin; Thalassemia; HPFH; Transcription; Chromatin remodeling
• ISSN: 1079-9796; 1096-0961
• DOI: 10.1016/j.bcmd.2006.07.003

The genetic mechanisms underlying the continued expression of the γ-globin genes during the adult stage in deletional hereditary persistence of fetal hemoglobin (HPFH) and δβ-thalassemias are not completely understood. Herein, we investigated the possible involvement of transcription factors, using the suppression subtractive hybridization (SSH) method as an initial screen to identify differentially expressed transcripts in reticulocytes from a normal and a HPFH-2 subject. Some of the detectable transcripts may participate in globin gene regulation. Quantitative real-time PCR (qRT-PCR) experiments confirmed the downregulation of ZHX2, a transcriptional repressor, in two HPFH-2 subjects and in a carrier of the Sicilian δβ-thalassemia trait. The chromatin remodeling factors ARID1B and TSPYL1 had a very similar pattern of expression with an incremental increase in HPFH and decreased expression in δβ-thalassemia. These differences suggest a mechanism to explain the heterocellular and pancellular distribution of fetal hemoglobin in δβ-thalassemia and deletional HPFH, respectively. Interestingly, α-globin mRNA levels were decreased, similar to β-globin in all reticulocyte samples analyzed.