Effective Dehairing Properties of Keratinase from Paenibacillus woosongensis TKB2 Obtained Under Solid State Fermentation

• Published in: Waste and biomass valorization Vol. 5; no. 1; pp. 97 - 107
• Main Authors: Paul, Tanmay, Das, Arpan, Mandal, Arpita, Jana, Arijit, Maity, Chiranjit, Adak, Atanu, Halder, Suman K., DasMohapatra, Pradeep K., Pati, Bikas R., Mondal, Keshab C.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.02.2014
• Subjects: Engineering; Environment; Environmental Engineering/Biotechnology; Industrial Pollution Prevention; Original Paper; Renewable and Green Energy; Waste Management/Waste Technology; Keratinase; Dehairing; Solid-state fermentation; Enzyme technology; Optimization; Enzyme production
• ISSN: 1877-2641; 1877-265X
• DOI: 10.1007/s12649-013-9217-z

Response surface methodology was employed for optimization of the culture conditions for keratinase production by a feather-degrading Paenibacillus woosongensis TKB2 under solid-state fermentation using chicken feather as substrate. The optimized culture conditions having 2.21 g of feather, with rice straw (2:1), moistened with distilled water (1:5, w/v adjusted to pH 8.5) and fermented for 72.07 h, resulted in 2.83-fold increased keratinase production compared to non-optimized condition. The enzyme efficiently dehaired goat hides within 14 h without the use of lime. The enzyme-dehaired goat skins were evaluated by mechanical, touch-visual and electron microscopy methods and were compared with conventional chemical treated skins. The results indicated that the enzyme treated dehaired skins exhibit similar or improved characteristics without damaging the collagen layer, which makes the crude keratinase a potential candidate for application in leather industry to avoid pollution problems associated with the use of chemicals.