Anti-adipogenic activity of blue mussel (Mytilus edulis) extract by regulation of 3T3-L1 adipogenesis through Wnt/β-catenin signaling pathway

• Published in: Food science and biotechnology Vol. 24; no. 1; pp. 315 - 321
• Main Authors: Shon, Myung-Soo, Kim, Si-Kyung, Song, Ji-Hye, Lee, Seung-Cheol, Kim, Gyo-Nam
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society of Food Science and Technology 01.02.2015; 한국식품과학회
• Subjects: Chemistry; Chemistry and Materials Science; Food Science; Mytilus edulis; Nutrition; Research Article; 식품과학; mussel; adipogenesis; Wnt/β-catenin; obesity
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-015-0042-y

The blue mussel ( Mytilus edulis ) is widely distributed in European waters but it is little consumed. Although several studies have been reported biological activities of mussels, the anti-adipogenic activity of blue mussel and the molecular mechanism in anti-obese activity remain unknown. Here, we prepared a water extract of blue mussel (BME) and its anti-adipogenic activity was assessed in 3T3-L1 cells. BME treatment dose-dependently inhibited 3T3-L1 lipid accumulation, as judged by Oil Red O staining and microscopic analysis. Consistent with this, BME treatment markedly suppressed mRNA expression levels of adipogenic genes, such as C/EBPβ, PPARγ, C/EBPα, and FAS, in 3T3-L1 mature adipocytes. BME-inhibited lipid accumulation in 3T3-L1 adipocytes was limited with treatment after adipocyte differentiation, particularly in the early and intermediate stages. We also showed that the Wnt/β-catenin signaling pathway is required in BME-inhibited 3T3-L1 adipogenesis. These finding may provide scientific evidence for the development of functional foods against obesity.